10745731001

β-Galactosidase

EIA grade

• Sinônimo(s): β galactosidase, β-galactosidase
• Número da licença da enzima: 3.2.1.23 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propriedades

• fonte biológica: Escherichia coli
• Nível de qualidade: 100
• Ensaio: (single peak, HPLC)
• Formulário: lyophilized
• atividade específica: 750-950 U/mg
• peso molecular: 540.000 kDa
• embalagem: pkg of 25 mg (approx. 100 mg lyophilizate)
• fabricante/nome comercial: Roche
• técnica(s): enzyme immunoassay: suitable
• cor: white
• pH: 7.3-7.7
• solubilidade: water: 20 mg/mL
• λmax: 405
• adequação: suitable for immunoassay
• nº de adesão NCBI: WP_050940144
• nº de adesão UniProt: P00722; ; ; ; P00722
• aplicação(ões): life science and biopharma
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C (−15°C to −25°C)
• Informações sobre genes: Escherichia coli ... lacZ(945006)

Descrição

Descrição geral

β-Galactosidase, EIA grade, is a lyophilizate from E. coli overproducer, consisting of enzyme protein, phosphate buffer, and sucrose. Substances which could interfere with the derivatization of NH₂ or SH groups (e.g., 2-mercaptoethanol, ammonium salts, primary amines etc.) have been removed.

The GLB1 (β-Galactosidase) gene is mapped to human chromosome 3p22.3.[1] The encoded protein belongs to glycosyl hydrolase 35 family and is localized to lysosomes.[2]

Aplicação

β-Galactosidase has been used as a standard to determine the absolute quantitation of LacZ protein.[3]

β-Galactosidase is used for labeling enzyme immunoassay techniques. β-Galactosidase can be coupled to other proteins via its SH-groups. The reconstituted solution can be used directly for conjugation without prior dialysis or gel permeation chromatography.

Ações bioquímicas/fisiológicas

β-Galactosidase catalyzes the hydrolysis of terminal β-galactose residue of ganglioside substrates, such as glycoproteins, sphingolipids, and keratan sulfate and other glycoconjugates.[2][4] This enzyme is associated with the mechanism of cell senescence and carcinogenesis.[2] Mutations in the gene result in gangliosidosis, an autosomal recessive disorder, characterized with defective lysosomal storage due to accumulation of substrates.[5][4] β-Galactosidase deficiency also causes Morquio B syndrome indicating skeletal abnormalities, short stature and increased excretion of keratan sulfate in urine.[4]

Qualidade

Purity: single peak (HPLC)

Sequência

Free Thiol Groups
The non-denatured, enzymatically-active preparation contains > 12 SH groups per molecule which are not involved in disulfide bridges and are freely accessible to coupling reagents in aqueous media (as assayed with Ellman′s reagent at +37 °C, acc. to Habeeb, 1972).

Absence of Enzyme Aggregates
The preparation contains < 3% dimers (HPLC, area %) and essentially no higher oligomers.

forma física

Lyophilizate, stabilized with phosphate buffer and sucrose. Note: Contains at least 12 free SH-groups/enzyme molecule; 5 mg approximately 20 mg lyophilizate; 25 mg approximately 100 mg lyophilizate.

Armazenamento e estabilidade

Store at -15–-25 °C. (sealed under nitrogen)

Nota de análise

Specific activity: approximately 750 - 950 U/mg enzyme protein ≅ approximately 150 - 250 U/mg lyophilizate (+37°C, 2-nitrophenyl-β-D-galactoside); approximately 250-400 U/mg enzyme protein ≅ approximately 60-100 U/mg lyophilizate (+37°C, 4-nitrophenyl-β-D-galactoside).

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash