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G3907

Millipore

Glutathione−Agarose

set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

Sinônimo(s):

GSH-agarose, S-linked glutathione agarose

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About This Item

Número MDL:
MFCD00131200
Código UNSPSC:
41106500
NACRES:
NA.56

R$ 3.490,00

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Nível de qualidade

200

Formulário

(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

classe(s) química(is) do analito

proteins (GST)

embalagem

set of 3 pre-packed columns (2.5 ml each)

técnica(s)

immunoprecipitation (IP): suitable
protein purification: suitable

Matriz

cross-linked 4% beaded agarose

temperatura de armazenamento

2-8°C

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Descrição geral

The resin in these columns consists of glutathione attached through the sulfur to epoxy activated, 4% cross-linked beaded agarose resulting in a 12 atom spacer. [1]

Aplicação

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of glutathione binding enzymes such as glutathione-S-transferase, glutathione peroxidase, and glyoxalase I.[2]
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4.[3] It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.[4]
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Ligação

Suspension of Product No. G 4510

forma física

1:1 suspension in a 0.5 M NaCl + 20% ethanol solutionl

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

180.1 °F

Ponto de fulgor (°C)

82.3 °C

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Certificados de análise (COA)

Lot/Batch Number
SLBR3849V
016K7056
074K7025

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L-Glutationa reduzida ≥98.0%

Sigma-Aldrich

G4251

L-Glutationa reduzida

Paul R Jenkins et al.
Bioorganic & medicinal chemistry, 16(16), 7728-7739 (2008-07-25)
We present the design, synthesis and biological activity of a library of substituted (biphenylcarbonyl)-tryptamine and (biphenylcarbonyl)-tetrahydro-beta-carboline compounds related to the natural product fascaplysin, as novel inhibitors of CDK4/cyclin D1. We show all these molecules, prepared using the Suzuki-Miyaura reaction, being
Carine Aubry et al.
Organic & biomolecular chemistry, 4(5), 787-801 (2006-02-24)
We present the design, synthesis, and biological activity of three classes of tryptamine derivatives, which are non-planar analogues of the toxic anti-cancer agent fascaplysin. We show these compounds to be selective inhibitors of CDK4 over CDK2, the most active compound
Diane M Kanter et al.
Nucleic acids research, 39(7), 2580-2592 (2010-11-27)
Sld2 is essential for the initiation of DNA replication, but the mechanism underlying its role in replication is not fully understood. The S-phase cyclin dependent kinase (S-CDK) triggers the association of Sld2 with Dpb11, and a phosphomimetic mutation of Sld2
F Toribio et al.
Journal of chromatography. B, Biomedical applications, 684(1-2), 77-97 (1996-09-20)
The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography
Dustin L Johnson et al.
Journal of bacteriology, 190(8), 2972-2980 (2008-02-19)
Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intracellular
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