Meio de Eagle modificado por Dulbecco - alto teor de glicose, With 4500 mg/L glucose, stable glutamine, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
Aplicação
O meio de Eagle modificado por Dulbecco (DMEM) é uma modificação do meio basal Eagle (BME) que contém concentrações quatro vezes maiores de aminoácidos e vitaminas. A formulação original contém 1000 mg/l de glicose e foi usada para cultura de células embrionárias de camundongo. Desde então, ele foi modificado de várias maneiras para dar suporte a culturas primárias de células de camundongo e frango, além de diversas células normais e transformadas. Cada um desses meios oferece uma combinação diferente de L-glutamina e piruvato de sódio. Além disso, os níveis de glicose foram elevados para 4500 mg/l, contribuindo para o nome "DMEM/alto".
Informações legais
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American journal of physiology. Heart and circulatory physiology, 320(3), H1156-H1169 (2021-01-16)
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