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Calcium Phosphate Transfection Kit

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

Sinônimo(s):

Gene delivery

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About This Item

Número CE:
233-140-8
Código UNSPSC:
12352200
NACRES:
NA.85

grau

for molecular biology

Nível de qualidade

200

forma

solution

uso

 kit sufficient for 160 transfections (6 cm dishes)
 kit sufficient for 400 transfections (3.5 cm dishes)
 kit sufficient for 80 transfections (10 cm dishes)

técnica(s)

transfection: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

Categorias relacionadas

Descrição geral

Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.

Aplicação

Calcium Phosphate Transfection Kit has been used:
  • to enable transfection
  • to transfect Hek293T cells
  • to transfect H29D cells

Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:

BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D

Características e benefícios

  • Suitable for transient and stable transfection
  • Reproducible for a wide range of cell types
  • Widely referenced
  • Inexpensive

Componentes

The Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)

Princípio

The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl2 solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.

Outras notas

The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H319

Classificações de perigo

Eye Irrit. 2

Código de classe de armazenamento

12 - Non Combustible Liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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M Wigler et al.
Cell, 14(3), 725-731 (1978-07-01)
Previous studies from our laboratories have demonstrated the feasibility of transferring the thymidine kinase (tk) gene from restriction endonuclease-generated fragments of herpes simplex virus (HSV) DNA to cultured mammalian cells. In this study, high molecular weight DNA from cells containing
Munjin Kwon et al.
Methods in molecular biology (Clifton, N.J.), 1018, 107-110 (2013-05-18)
The calcium phosphate transfection is a widely used method for introducing foreign DNA plasmids into cells. Mechanisms underlying this transfection method are not yet defined; however, DNA-calcium phosphate precipitates are internalized by the cells and DNA is efficiently expressed in
A new technique for the assay of infectivity of human adenovirus 5 DNA.
F L Graham et al.
Virology, 52(2), 456-467 (1973-04-01)
Denise A Carbonaro et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 22(3), 607-622 (2013-11-22)
Gene transfer into autologous hematopoietic stem cells by γ-retroviral vectors (gRV) is an effective treatment for adenosine deaminase (ADA)-deficient severe combined immunodeficiency (SCID). However, current gRV have significant potential for insertional mutagenesis as reported in clinical trials for other primary
Paolo Conrotto et al.
Molecular oncology, 5(6), 527-537 (2011-09-02)
The transcription factor SOX11 is a novel diagnostic marker for mantle cell lymphoma (MCL), distinguishing this aggressive tumor from potential simulators. Recent data also show that the level of SOX11 correlates to in vitro growth properties in MCL, as well
Exibir todos os documentos relacionados

Artigos

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Protocolos

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Conteúdo relacionado

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

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