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Documentos Principais

51456

Sigma-Aldrich

Ureia

BioUltra, for molecular biology, 99% (T)

Sinônimo(s):

Carbamida, Carbonil diamida

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500 G
R$ 1.307,00
2.5 KG
R$ 2.642,00

R$ 1.307,00


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500 G
R$ 1.307,00
2.5 KG
R$ 2.642,00

About This Item

Fórmula linear:
NH2CONH2
Número CAS:
Peso molecular:
60.06
Beilstein:
635724
Número CE:
Número MDL:
Código UNSPSC:
12352111
ID de substância PubChem:
NACRES:
NA.25

R$ 1.307,00


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grau

Molecular Biology
for molecular biology

linha de produto

BioUltra

Ensaio

99% (T)

Formulário

powder or crystals

Impurezas

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.1% biuret
<0.005% formaldehyde (HCHO)

resíduo de ignição

≤0.01% (as SO4)

perda

≤0.1% loss on drying, 20 °C (HV)

pH

8.0-10.0 (20 °C, 8 M in H2O)

pf

132-135 °C (lit.)

solubilidade

H2O: 8 M at 20 °C, clear, colorless

densidade

1.335 g/mL at 25 °C (lit.)

traços de ânion

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤10 mg/kg

traços de cátion

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

cadeia de caracteres SMILES

NC(N)=O

λ

8 M in H2O

Absorção UV

λ: 260 nm Amax: ≤0.03
λ: 280 nm Amax: ≤0.02

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

chave InChI

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Informações sobre genes

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Aplicação

Usado na desnaturação de proteínas e como agente suave de solubilização para proteínas insolúveis ou desnaturadas. Útil para renaturar proteínas de amostras já desnaturadas com cloreto de guanidina 6 M, como corpos de inclusão. Pode ser usado com cloridrato de guanidina e ditiotreitol (DTT) para renaturação de proteínas desnaturadas para sua forma nativa ou ativa.

Outras notas

Denaturing reagent[1]; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides[2][3][4]

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Certificados de análise (COA)

Lot/Batch Number

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Supelco

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Ureia

R. Poulson et al.
The Ribonucleic Acids, 333-333 (1977)
Fractionation of low molecular weight DNA or RNA in polyacrylamide gels containing 98% formamide or 7 M urea.
T Maniatis et al.
Methods in enzymology, 65(1), 299-305 (1980-01-01)
A Paulus et al.
Electrophoresis, 14(1-2), 27-35 (1993-01-01)
DNA digest analysis with polymer-filled capillaries in capillary electrophoresis is described. The samples analyzed consisted of commercially available standards including a 100 base pair ladder with repeating units up to 2000 base pairs. Three DNA digests covered the most common
R.C. Ogden et al.
Methods in Enzymology, 152, 66-66 (1987)
Florin Sasarman et al.
Human molecular genetics, 24(10), 2841-2847 (2015-02-06)
Addition of the trinucleotide cytosine/cytosine/adenine (CCA) to the 3' end of transfer RNAs (tRNAs) is essential for translation and is catalyzed by the enzyme TRNT1 (tRNA nucleotidyl transferase), which functions in both the cytoplasm and mitochondria. Exome sequencing revealed TRNT1

Protocolos

An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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