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45260

Sigma-Aldrich

Eosin B

for microscopy (Fl., Hist.), adsorption and fluorescent indicator

Sinônimo(s):

4′,5′-Dibromo-2′,7′-dinitrofluorescein disodium salt, Acid Red 91, Eosin Scarlet, Eosin bluish, Saffrosine

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About This Item

Fórmula linear:
C20H6N2O9Br2Na2
Número CAS:
548-24-3
Peso molecular:
624.06
Número do índice de cores:
45400
Beilstein:
4121964
Número CE:
208-943-1
Número MDL:
MFCD00005041
Código UNSPSC:
12171500
ID de substância PubChem:
329756626
NACRES:
NA.47

grau

adsorption and fluorescent indicator
for microscopy (Fl., Hist.)

técnica(s)

titration: suitable

λmax

509-524 nm

cadeia de caracteres SMILES

[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O

InChI

1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2

chave InChI

GYYTYUGGVBYJHE-UHFFFAOYSA-L

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Descrição geral

Eosin B is a fluorescent red dye that is used in microscopy to visualise cytoplasm and proteins like collagen.

Aplicação

Eosin B is an adsorption and fluorescent indicator in microscopy.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

Certificados de análise (COA)

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Kristen M Massimine et al.
Antimicrobial agents and chemotherapy, 50(9), 3132-3141 (2006-08-31)
4',5'-Dibromo-2',7'-dinitrofluorescein, a red dye commonly referred to as eosin B, inhibits Toxoplasma gondii in both enzymatic and cell culture studies with a 50% inhibitory concentration (IC(50)) of 180 microM. As a non-active-site inhibitor of the bifunctional T. gondii dihydrofolate reductase-thymidylate
A A Waheed et al.
Journal of biochemical and biophysical methods, 42(3), 125-132 (2000-03-29)
Eosin B is used to estimate proteins above 1 microg/ml concentration [Waheed AA, Gupta, PD. Anal. Biochem. 1996:233:249-256; Waheed AA, Gupta PD. J. Biochem. Biophys. Meth. 1996;33:187-196]. In the present report we describe a method for estimating submicrogram quantities of
A A Waheed et al.
Journal of biochemical and biophysical methods, 33(3), 187-196 (1996-12-30)
We describe a detailed procedure for estimating a wide range of proteins by the eosin B dye method. At acidic pH, eosin B binds to proteins and absorption of the protein-dye complex at 536-544 nm is proportional to the concentration
Y M Cheng et al.
Neuroscience, 151(4), 1084-1098 (2008-01-19)
We examined the potential contribution of a voltage-gated proton conductance (gH+) to acid extrusion from cultured postnatal rat hippocampal neurons. In neurons loaded with Ca2+- and/or pH-sensitive fluorophores, transient exposures to 25-139.5 mM external K+ (K+o) or 20 microM veratridine
Rebecca Pastrana-Mena et al.
The Journal of biological chemistry, 285(35), 27045-27056 (2010-06-25)
Malaria parasites contain a complete glutathione (GSH) redox system, and several enzymes of this system are considered potential targets for antimalarial drugs. Through generation of a gamma-glutamylcysteine synthetase (gamma-GCS)-null mutant of the rodent parasite Plasmodium berghei, we previously showed that
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