PPB019

Bis-Tris Bicine EDTA buffer

pH 7.2, pHast Pack^™, powder

• Sinônimo(s): 1X Bis-Tris transfer buffer, Bis-Tris
• Código UNSPSC: 41105319
• NACRES: NA.25

Propriedades

• linha de produto: pHast Pack^™
• Nível de qualidade: 100
• Formulário: powder
• condição de armazenamento: (Tightly closed. Dry)
• pH: 7.2
• traços de cátion: Pb: ≤5 ppm
• atividade externa: DNAse, free; NICKase, free; Protease, free; RNAse, free
• temperatura de armazenamento: room temp

Descrição

Descrição geral

Bis-Tris Bicine EDTA buffer or Bis-Tris buffer (pH 7.2) contains Bis-Tris and Bicine, zwitterionic buffer components that form strong bonds with common metals. In combination with ethylenediaminetetraacetic acid (EDTA), a metal chelator, that binds divalent cations. This buffer is commonly used as a transfer buffer in western blot to transfer proteins from the gel to the membrane.

Aplicação

Bis-Tris buffer may be used in western blot as a transfer buffer.

Características e benefícios

• Ready-to-use buffer
• No measuring and pH adjustment needed
• Certified quality for reproducibility
• Minimizes exposure to toxic chemicals
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Embalagem

Foil pouches

Reconstituição

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1X solution containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, pH 7.2 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Armazenamento e estabilidade

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Outras notas

For additional information on our range of Biochemicals, please complete this form.

Informações legais

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Pictogramas: GHS05
• Palavra indicadora: Danger
• Frases de perigo: H318
• Declarações de precaução: P280 - P305 + P351 + P338
• Classificações de perigo: Eye Dam. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3