PPB001

Tris Buffered Saline

powder, pH 8.0, pHast Pack^™

• Sinônimo(s): 1X TBS wash buffer, TBS
• Número MDL: MFCD00132476
• Código UNSPSC: 12161700
• NACRES: NA.21

Propriedades

• product name: Tris Buffered Saline, pH 8.0, pHast Pack^™, powder
• linha de produto: pHast Pack^™
• forma: powder
• condição de armazenamento: (Tightly closed. Dry)
• Impurezas: ≤5 ppm Heavy Metals (lead)
• cor: white
• pH: 8.0
• solubilidade: soluble, clear, colorless
• traços de cátion: Fe: <10 ppm
• atividade externa: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• temperatura de armazenamento: room temp

Descrição

Descrição geral

Tris Buffered Saline, pH 8.0 is a commonly used wash buffer that is isotonic and nontoxic and suitable for use in cell culture and electrophoresis applications.

Aplicação

Tris-buffered saline is intended to use for:

• Sample preparation
• ELISA
• Western Blot and other protein applications
• Cell culture (recommend to sterile filter)
• Immunohistochemistry
• Resuspension and sonication of cells

Características e benefícios

• Ready-to-use buffer – fast and easy to prep
• No need to measure or adjust pH, simply add water
• Saves time and minimizes the effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Embalagem

Foil pouches

Reconstituição

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 50mM Tris, 138mM NaCl, 2.7mM KCl, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Armazenamento e estabilidade

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Outras notas

For additional information on our range of Biochemicals, please complete this form.

Informações legais

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable