P3504

Ponceau S

Powder

• Sinônimo(s): 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
• Fórmula empírica (Notação de Hill): C₂₂H₁₂N₄Na₄O₁₃S₄
• Número CAS: 6226-79-5
• Peso molecular: 760.57
• Número do índice de cores: 27195
• Número CE: 228-319-2
• Número MDL: MFCD00003892
• Código UNSPSC: 12171500
• ID de substância PubChem: 24898418
• NACRES: NA.47

Propriedades

• product name: Ponceau S, BioReagent, suitable for electrophoresis
• linha de produto: BioReagent
• Nível de qualidade: 200
• forma: powder
• técnica(s): electrophoresis: suitable
• cor: red to very dark red
• solubilidade: H₂O: 1 mg/mL
• ε (coeficiente de extinção): ≥330000 at 518-522 nm in deionized water at 0.015 g/L
• aplicação(ões): diagnostic assay manufacturing; hematology; histology
• temperatura de armazenamento: room temp
• cadeia de caracteres SMILES: [Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
• chave InChI: KQHKSGRIBYJYFX-WNDMICSESA-J

Descrição

Descrição geral

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.
The following are two common stain formulations:
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Aplicação

Ponceau S has been used for membrane staining (Western blotting.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Gloves