03450
N-(3-dimetilaminopropil)-N′-etilcarbodiimida
purum, ≥98.0% (AT)
Sinônimo(s):
N-etil-N′-(3-dimetilaminopropil)carbodiimida, EDAC, EDC, WSC
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1 G
R$ 212,00
5 G
R$ 677,00
25 G
R$ 2.212,00
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1 G
R$ 212,00
5 G
R$ 677,00
25 G
R$ 2.212,00
About This Item
Fórmula empírica (Notação de Hill):
C8H17N3 · HCl
Número CAS:
Peso molecular:
191.70
Beilstein:
5764110
Número CE:
Número MDL:
Código UNSPSC:
12352111
ID de substância PubChem:
NACRES:
NA.31
Produtos recomendados
grau
purum
Nível de qualidade
Ensaio
≥98.0% (AT)
Formulário
powder
pf
110-115 °C (lit.)
110-115 °C
solubilidade
H2O: soluble 1 gm/10 ml, clear to very slightly hazy, colorless to very faintly yellow
aplicação(ões)
microbiology
temperatura de armazenamento
−20°C
cadeia de caracteres SMILES
Cl.CCN=C=NCCCN(C)C
InChI
1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
chave InChI
FPQQSJJWHUJYPU-UHFFFAOYSA-N
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Descrição geral
1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC HCl), commonly known as EDAC, is a crucial water-soluble reagent widely employed in chemical and biochemical research, particularly for its proficiency in mediating amide bond formation. Notably effective in peptide synthesis, EDAC HCl demonstrates efficiency in coupling amino acids through their carboxyl and amine groups, enabling the tailored creation of peptides with specific sequences and functionalities. Extending its utility, EDAC HCl plays a pivotal role in the construction of immunogens by covalently attaching haptens—small immune-response eliciting molecules—to carrier proteins, a critical aspect in vaccine research.
The adaptability of EDAC HCl encompasses nucleic acid modification, permitting the selective labeling of DNA and RNA through their 5′ phosphate groups. This capacity contributes significantly to the visualization, tracking, and analytical aspects of these fundamental molecules, thereby advancing nucleic acid research. Furthermore, EDAC HCl functions as a biomolecule bridge, acting as a crosslinker connecting amine-reactive NHS-esters of biomolecules to carboxyl groups.
This feature proves invaluable in protein conjugation, facilitating the development of hybrid molecules with distinct properties and functions. The underlying reaction mechanism involves EDAC HCl′s interaction with a carboxyl group, forming an unstable intermediate actively seeking an amine partner. The delicate equilibrium of this reaction underscores the necessity for optimizing conditions to ensure efficient conjugation. The assistance of N-hydroxysuccinimide (NHS) enhances the capabilities of EDAC HCl by stabilizing the intermediate and enabling two-step conjugation procedures, affording greater flexibility and control, particularly in the manipulation of complex biomolecular structures.
The adaptability of EDAC HCl encompasses nucleic acid modification, permitting the selective labeling of DNA and RNA through their 5′ phosphate groups. This capacity contributes significantly to the visualization, tracking, and analytical aspects of these fundamental molecules, thereby advancing nucleic acid research. Furthermore, EDAC HCl functions as a biomolecule bridge, acting as a crosslinker connecting amine-reactive NHS-esters of biomolecules to carboxyl groups.
This feature proves invaluable in protein conjugation, facilitating the development of hybrid molecules with distinct properties and functions. The underlying reaction mechanism involves EDAC HCl′s interaction with a carboxyl group, forming an unstable intermediate actively seeking an amine partner. The delicate equilibrium of this reaction underscores the necessity for optimizing conditions to ensure efficient conjugation. The assistance of N-hydroxysuccinimide (NHS) enhances the capabilities of EDAC HCl by stabilizing the intermediate and enabling two-step conjugation procedures, affording greater flexibility and control, particularly in the manipulation of complex biomolecular structures.
Aplicação
Ações bioquímicas/fisiológicas
Reagente de condensação solúvel em água. O EDAC, de modo geral, é utilizado como agente de ativação de grupos carboxílicos para ligação de amidas com aminas primárias. Além disso, ele reage com grupos fosfato. O EDAC foi usado, por exemplo, na síntese de peptídeos; reticulação de proteínas com ácidos nucleicos; e no preparo de imunoconjugados. Tipicamente, o EDAC é utilizado na faixa de pH de 4,0 a 6,0 sem tampões. Em particular, tampões com amina e carboxilato devem ser evitados.
N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide is a water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines.[3] Additionally, it reacts with phosphate groups. It has been utilized in peptide synthesis, crosslinking proteins to nucleic acids as well as preparation of immunoconjugates.[4]
Características e benefícios
Versatile and adaptable for a wide variety of laboratory and research applications
Outras notas
For additional information on our range of Biochemicals, please complete this form.
produto comparável
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
Órgãos-alvo
Stomach,large intestine,lymph node
Código de classe de armazenamento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
dust mask type N95 (US), Eyeshields, Gloves
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