11772457001
Roche
TUNEL AP
sufficient for 70 tests, solution, pkg of 3.5 mL
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About This Item
Código UNSPSC:
41105600
Produtos recomendados
Formulário
solution
uso
sufficient for 70 tests
embalagem
pkg of 3.5 mL
fabricante/nome comercial
Roche
Condições de expedição
wet ice
temperatura de armazenamento
2-8°C
Descrição geral
TUNEL AP is an alkaline phosphatase-labeled antibody used for the in situ detection of apoptosis (programmed cell death) with the TUNEL reaction followed by transmission light microscopy.
The tailing reaction using TdT, also named ISEL (in situ end labeling) or TUNEL (TdT-mediated dUTP nick end labeling), has several advantages in comparison to the in situ nick translation (ISNT) using DNA polymerase:
- Label intensity of apoptotic cells is higher with TUNEL compared to ISNT, resulting in an increased sensitivity.
- Kinetics of nucleotide incorporation is very rapid with TUNEL compared to the ISNT.
- TUNEL preferentially labels apoptotic cells compared to necrotic cells.
Aplicação
TUNEL AP is an antibody that is used to convert fluorescence-based TUNEL assays into colorimetric assays suited for light microscopy. The conversion is performed by binding of an anti-fluorescein antibody to FITC-dUTP. The antibody is labeled with alkaline phosphatase (AP). The AP is visualized with a precipitating substrate, such as Fast Red or NBT/BCIP.
Componentes
Anti-fluorescein antibody, Fab fragment from sheep, conjugated with alkaline phosphatase (AP). Ready-to-use solution.
Outras notas
For life science research only. Not for use in diagnostic procedures.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Skin Sens. 1
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
No data available
Ponto de fulgor (°C)
No data available
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