11699105001

PCR Buffer Without MgCl₂, 10x concentrated

solution, pkg of 3 × 1 mL

• Sinônimo(s): buffer
• Código UNSPSC: 41105600

Propriedades

• Formulário: solution
• Nível de qualidade: 100
• embalagem: pkg of 3 × 1 mL
• fabricante/nome comercial: Roche
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

The PCR buffer without MgCl₂, in combination with the MgCl₂ stock solution, is used for the individual adjustment of the Mg²⁺ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl₂ stock solution.

Aplicação

The PCR buffer without MgCl₂ has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).

forma física

Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

Outras notas

For general laboratory use.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash