M8787

Magnesium chloride solution

PCR Reagent, 25 mM MgCI₂ solution for PCR

• Sinônimo(s): PCR optimization
• Fórmula linear: MgCl₂
• Número CAS: 7786-30-3
• Peso molecular: 95.21
• Número MDL: MFCD00011106
• Código UNSPSC: 12352302
• ID de substância PubChem: 57654422
• NACRES: NA.52

Propriedades

• Nível de qualidade: 200; 300
• Formulário: liquid
• embalagem: vial of 1.5 mL
• concentração: 25 mM±1 mM
• técnica(s): PCR: suitable
• cor: colorless
• aplicação(ões): agriculture; diagnostic assay manufacturing
• atividade externa: DNase, RNase, none detected
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: Cl[Mg]Cl
• InChI: 1S/2ClH.Mg/h2*1H;/q;;+2/p-2
• chave InChI: TWRXJAOTZQYOKJ-UHFFFAOYSA-L

Descrição

Descrição geral

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Aplicação

Magnesium chloride solution has been used as a component of the:

• amplification mixture for polymerase chain reaction (PCR)[1]
• amplification mixture in an inter simple sequence repeats (ISSR)-PCR[2]
• reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)[3]
• PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA[4]
• reaction mix for RT-PCR to assess the gene expression[5]

Adequação

Suitable for optimization of polymerase chain reactions.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)