11667475001
Roche
Anti-HA-Peroxidase
from mouse IgG2bκ
Sinônimo(s):
antibody
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About This Item
Código UNSPSC:
12352200
Produtos recomendados
fonte biológica
mouse
conjugado
peroxidase conjugate
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
12CA5, monoclonal
Ensaio
≥95% (HPLC and SDS-PAGE)
Formulário
solution
embalagem
pkg of 50 μg (500 μl)
fabricante/nome comercial
Roche
concentração
0.1 mg/mL
Isotipo
IgG2bκ
sequência de epítopo
YPYDVPDYA
temperatura de armazenamento
−20°C
Descrição geral
Anti-HA-Peroxidase is a monoclonal antibody to the HA peptide, conjugated to peroxidase. Anti-HA-Peroxidase recognizes the HA nonapeptide sequence (YPYDVPDYA), derived from the human influenza virus hemagglutinin protein (amino acids 98-106). The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".
Especificidade
Anti-HA-Peroxidase recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
The antibody reacts with the HA epitope, a nonapeptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein.
Imunogênio
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Aplicação
- Use Anti-HA-Peroxidase for the immunochemical detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:
- Dot blot
- Western blot
Note: For experiments in which sensitivity is not critical, use Anti-HA-Peroxidase (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity.
Qualidade
Each lot of Anti-HA antibody is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation.
Nota de preparo
Stabilizers: BSA, sucrose, 0.02% thymol
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
Working solution: 20-fold dilution of Western Blocking Reagent in 1x PBS.
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
- Western blot: 0.1 μg/ml
Working solution: 20-fold dilution of Western Blocking Reagent in 1x PBS.
Outras notas
For life science research only. Not for use in diagnostic procedures.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
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