11585029001

Cloreto de nitrotetrazólio azul

crystals, pkg of 5 g

• Sinônimo(s): Cloreto de p-nitroazul de tetrazólio, p-Nitrotetrazólio azul, Cloreto de 2,2′-bis(4-nitrofenil)-5,5′-difenil-3,3′-(3,3′-dimetoxi-4,4′-difenileno)ditetrazólio, Cloreto de 3,3′-(3,3′-dimetoxi-4,4′-bifenileno)bis[2-(4-nitrofenil)-5-fenil-2H-tetrazólio], NBT, Nitro BT
• Fórmula linear: C₄₀H₃₀N₁₀O₆ · 2Cl
• Número CAS: 298-83-9
• Peso molecular: 817.64
• Beilstein: 4115923
• Número MDL: MFCD00012159
• Código UNSPSC: 41116100
• ID de substância PubChem: 329749491

Propriedades

• descrição: 4-Nitro blue tetrazolium chloride, crystals
• Nível de qualidade: 100
• Formulário: crystals
• embalagem: pkg of 5 g
• fabricante/nome comercial: Roche
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• chave InChI: FSVCQIDHPKZJSO-UHFFFAOYSA-L

Descrição

Descrição geral

NBT is soluble in water, aqueous buffers, and dimethylformamide.

Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology.[1] In immunolabeling technique, 4-Nitro blue tetrazolium (NBT) serves as an oxidant. The hydrolysis of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) by alkaline phosphatase gives rise to leucoindigo, which is then being reduced by NBT to form an insoluble blue BCI.[2] Certain cells reduce NBT, which is a colorless/yellow dye to blue or black formazan crystals. Due to this property, NBT is prefered in redox histochemistry and in biochemical applications.[1] It is formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.[3]

Aplicação

• Colony and plaque hybridization
• Immunohistocytochemistry
• In situ hybridization[4]
• Northern blot
• Southern blot
• Western blot

NBT has been used in in situ hybridization.[5]

Nota de preparo

Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.

Nota de análise

Absorption: NBT has its maximum absorption at 260 nm [in water],
and the final product formazan has its maximum absorption at 605 nm [in nitrobenzole].

Performance-controlled in the LDH assay.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H302,H315,H319,H335
• Declarações de precaução: P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233
• Classificações de perigo: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash