11493027001
Roche
IsoStrip™ Mouse Monoclonal Antibody Isotyping Kit
sufficient for 10 tests
Sinônimo(s):
hybridoma subtyping
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About This Item
Código UNSPSC:
23201100
Produtos recomendados
uso
sufficient for 10 tests
Nível de qualidade
fabricante/nome comercial
Roche
temperatura de armazenamento
2-8°C
Categorias relacionadas
Descrição geral
IsoStrip Mouse Monoclonal Antibody Isotyping Kit is a simple, three-step kit for the rapid characterization of mouse monoclonal antibodies.
Contents
Contents
- Development Tubes, contain lyophilized latex beads, precoated with anti-mouse-Ig antibodies
- Isotyping Strips, precoated with subclass- and light-chain-specific anti-mouse-Ig antibodies
Especificidade
Anti-mouse antibodies, bound to the latex beads, will react with any mouse monoclonal antibody regardless of its isotype.Goat anti-mouse antibodies, immobilized to the strip, specifically bind to each of the common mouse antibody isotypes (IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA), and to the light chains.
Specificity for all specific antibodies beside IgML at 1.0 μg/ml: ≥ 2
IgM Lambda, at 1.0 μg/ml: ≥ 1
Specificity for all specific antibodies beside IgML at 1.0 μg/ml: ≥ 2
IgM Lambda, at 1.0 μg/ml: ≥ 1
Aplicação
The kit is used for the rapid identification of class, subclass, and light-chain types of mouse monoclonal antibodies (immunoglobulins).
Características e benefícios
- Reliable: Yields comparable results to the standard ELISA assay
- Fast: 5 minutes from start to finish
- Easy: Only 3 steps
- Economical: No equipment or additional reagents required
- Highly specific: No crossreactivity with FBS
Embalagem
1 kit containing 2 components.
Especificações
Assay time: 5 minutes
Sample material: Hybridoma supernatant, purified mouse monoclonal antibodies, ascites
Sample material: Hybridoma supernatant, purified mouse monoclonal antibodies, ascites
Nota de preparo
Working solution: Dilute Sample
- Remove the desired number of isotyping strips from the canister. Remove the caps from an equal number of development tubes.
The tubes may be labeled with a pencil or felt-tipped lab marker for easy identification. - Dilute a sample containing the mouse monoclonal antibody in 1% BSA/ phosphate-buffered saline (PBS), pH 7.2 to 7.6.
Culture supernatant samples should be diluted 1:10 to 1:100. Ascites samples should be diluted 1:20,000. These are recommended dilutions and may vary depending on the concentration of antibody in your sample. In our experience, a monoclonal antibody
concentration of 0.1 to 1 g/ml of diluted sample gives the best results. 150 ml of this diluted sample will be added to the development tube.
Outras notas
For life science research only. Not for use in diagnostic procedures.
Informações legais
IsoStrip is a trademark of Roche
Somente componentes do kit
Nº do produto
Descrição
- Development Tubes, containing lyophilized latex beads, precoated with anti-mouse-Ig antibodies
- Isotyping Strips, precoated with subclass- and light-chain-specific anti-mouse-Ig antibodies
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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