11388908910
Roche
Biotin-16-UTP
pkg of 25 μL (250 nmol; 10mM)
Sinônimo(s):
Biotin-16-UTP, biotin
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About This Item
Código UNSPSC:
41116100
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
Ensaio
97.7% (HPLC)
Nível de qualidade
Formulário
solution
peso molecular
Mr 987.5 (biotin-16-UTP-Li4)
embalagem
pkg of 25 μL (250 nmol; 10mM)
fabricante/nome comercial
Roche
cor
colorless
solubilidade
water: miscible
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Categorias relacionadas
Descrição geral
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling.[1] Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
Aplicação
Biotin-labeled RNA can be used as a hybridization probe for:
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
Qualidade
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
forma física
Biotin-16-UTP, tetralithium salt, 10mM solution
Outras notas
For life science research only. Not for use in diagnostic procedures.
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
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