11062590001

DNA Molecular Weight Marker VI

pkg of 50 μg (in 200 μl), solution

• Sinônimo(s): DNA marker
• Código UNSPSC: 41105335

Propriedades

• Formulário: solution
• Nível de qualidade: 100
• embalagem: pkg of 50 μg (in 200 μl)
• fabricante/nome comercial: Roche
• concentração: 250 μg/mL
• cor: colorless
• solubilidade: water: miscible
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

DNA Molecular Weight Marker VI is a fragment mixture prepared by cleavage of pBR328 DNA with restriction endonucleases Bgl I and pBR328 DNA digested with Hinf I. It has a size range of 0.15 to 2.1kbp.
The product is also available as DNA Molecular Weight Marker VI, Digoxigenin-labeled.

Especificidade

Specificity: Product has 5′-phosphorylated ends (restriction fragments).
Restriction site: Claevage sites and corresponding fragment lengths of pBR328:
- Bgl I cut sites at: 935, 1169, 2399, 4575, 4809 => creating following fragment sizes, respectively: 234 bp, 1230 bp, 2176 bp, 234 bp, 1033 bp.
- Hinf I cut sites at: 632, 852, 1006, 1304, 1757, 2274, 4040, 4434, 4732, 4886. =>
creating following fragment sizes, respectively: 220 bp, 154 bp, 298 bp, 453 bp, 517 bp,
1766 bp, 394 bp, 298 bp, 154 bp, 653 bp

Aplicação

DNA Molecular Weight Marker VI is used for the size determination of DNA in agarose gels.[1] It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

• Restriction digests[2]
• PCR[3]
• RT-PCR

Sequência

As determined by computer analysis of the pBR328 sequence, the mixture contains 15 DNA fragments with the following base pair lengths (1 base pair = 660 daltons) occuring once: 220, 394, 453, 517, 653, 1033, 1230, 1766, 2176 and fragments 154,234, and 298 occuring twice as a result of the digestion of pBR328 with both Bgl I and Hinf I.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Definição da unidade

50 μg = 1A₂₆₀ unit

forma física

Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).

Nota de preparo

Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash