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Roche

CPRG

Chlorophenol red-β-D-galactopyranoside

Sinônimo(s):

Chlorophenol Red-β-D-galactopyranoside, chlorophenol red-β-d-galactopyranoside, CPRG, Chlorophenol Red-β-D-galactoside

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About This Item

Fórmula empírica (Notação de Hill):
C25H22Cl2O10S
Número CAS:
Peso molecular:
585.41
Número MDL:
Código UNSPSC:
12352204
ID de substância PubChem:

Nível de qualidade

Ensaio

96% (HPLC)

forma

powder

embalagem

pkg of 250 mg

fabricante/nome comercial

Roche

condição de armazenamento

protect from light

faixa de pH

6-8

Condições de expedição

dry ice

temperatura de armazenamento

−20°C (−15°C to −25°C)

cadeia de caracteres SMILES

OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1

chave InChI

YOUWVNCQJOMMEU-AETRGLENSA-N

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Descrição geral

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength dye which is commonly used for colorimetric assays. The reaction product has a dark red color and is soluble in water. It can be evaluated photometrically at 574 or 578 nm.
The chemical formula on the label represents the monosodium salt.

Aplicação

CPRG has been used as a substrate for β-galactosidase assay.
Colorimetric determination of β-Gal activity in cell extracts and ELISA.

Ações bioquímicas/fisiológicas

Chlorophenol red-β-D-galactopyranoside (CPRG) might possess oestrogenic activity.

Qualidade

Contaminant: <0.1% chlorophenol red

Sequência

CPRG is an approximately 1:1 mixture of the isomers.

Nota de preparo

Working concentration: 5 mmol/l
Storage conditions (working solution): Reconstituted solution is stable for one day at 2 to 8 °C

Armazenamento e estabilidade

Store powder dry at -15 to -25 °C; stock solutions at 2–8 °C protected from light.

Outras notas

For life science research only. Not for use in diagnostic procedures.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

No data available

Ponto de fulgor (°C)

No data available


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Mallika Ghosh et al.
Bio-protocol, 2(24), e305-e305 (2012-12-20)
Antigen cross presentation is important for effective immune responses to tumors and viral infections. Dendritic cells are professional antigen presenting cells and are unique in their ability to cross-present exogenous antigens on MHC class I molecules and activate antigen specific
Els Vanderperren Wim Demaré Ronny Blust Kris Cooreman Peter Bossier
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, 6(5), 375-380 (2001-01-01)
The finding that a variety of chemicals display oestrogenic activity has resulted in the development of in vitro and in vivo assays to assess oestrogenic activity. One such assay, the yeast oestrogen assay (YES) makes use of recombinant yeast cells
Matthias A Fügi et al.
Journal of lipid research, 55(5), 929-938 (2014-03-15)
Sterols are an essential class of lipids in eukaryotes, where they serve as structural components of membranes and play important roles as signaling molecules. Sterols are also of high pharmacological significance: cholesterol-lowering drugs are blockbusters in human health, and inhibitors
Clémence Sicard et al.
Analytical and bioanalytical chemistry, 406(22), 5395-5403 (2014-06-29)
We report on a new fluorimetric assay for β-galactosidase (β-gal) and faecal coliform bacteria that utilizes a long-wavelength dye, chlorophenol red-β-D-galactopyranoside (CPRG), that has been widely used for colorimetric assays. The novel feature of this new assay is the unexpected
Evan Amalfitano et al.
Nature communications, 12(1), 724-724 (2021-02-03)
Recent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential to provide decentralized and low-cost molecular diagnostics. However, it remains a challenge to deliver this sensing capacity into the hands of users in a

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