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Documentos Principais

SCT106

Sigma-Aldrich

BioTracker 490 Green Cytoplasmic Membrane Dye

Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.

Sinônimo(s):

Cytoplasmic membrane dye, Live cell imaging probe

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.47
Preço e disponibilidade não estão disponíveis no momento.

técnica(s)

cell based assay: suitable

método de detecção

fluorometric

Condições de expedição

ambient

Descrição geral

Lipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.

Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.

Spectral Properties
Absorbance: 484nm
Emission: 501nm

Aplicação

Live cell fluorescent imaging
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Qualidade

Spectral Properties
Absorbance: 484nm
Emission: 501nm

forma física

Liquid

Armazenamento e estabilidade

Store BioTracker 490 Green Cytoplasmic Membrane Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogramas

FlameExclamation mark
GHS02,GHS07

Palavra indicadora

Danger

Frases de perigo

H225,H319

Classificações de perigo

Eye Irrit. 2 - Flam. Liq. 2

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

55.4 °F

Ponto de fulgor (°C)

13 °C

Certificados de análise (COA)

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Questions

1–2 of 2 Questions  

  1. Biotracker 490 형광체의 Excitation, Emission curver를 알고싶습니다.

    1 answer

    1. BioTracker 490 Green Cytoplasmic Membrane Dye exhibits absorbance at 484 nm and emission at 501 nm. The image below displays the excitation and emission spectra of BioTracker 490 Green.

      Helpful?

  2. I am already using the BioTracker NIR750 and I am very satisfied. For this product I don't see the Data Sheet. At which concentration is it delivered? Which is the optimal final concentration to use? For how many round of cell division can be detected?

    1 answer

    1. The product is supplied as a 200 X solution. The specific formulation is considered proprietary. The dye is added at a rate of 5 ul per mL of culture medium. The lipophilic carbocyanine dyes have been used to stain neuronal cells in culture for several weeks, and in vivo for up to a year. The dyes do not appreciably affect cell viability and do not readily transfer between cells with intact membranes which allows for use in cell migration and tracking studies of mixed cell populations. The stability of labeling varies based on cell types and the rate of membrane turnover or cell division. Please see the link below to review a product Certificate of Analysis which serves as the datasheet:
      https://www.emdmillipore.com/US/en/product/BioTracker-490-Green-Cytoplasmic-Membrane-Dye,MM_NF-SCT106#documentation

      Helpful?

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