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PKH26GL

Sigma-Aldrich

Kit ligante de células com fluorescente vermelho PKH26 para a marcação geral da membrana celular

Distributed for Phanos Technologies

Sinônimo(s):

Red PKH membrane dye

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1 KIT
R$ 9.352,00

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1 KIT
R$ 9.352,00

About This Item

Código UNSPSC:
12352207
NACRES:
NA.32

R$ 9.352,00

Check Cart for Availability

embalagem

pkg of 1 kit

Nível de qualidade

200

fabricante/nome comercial

Distributed for Phanos Technologies

condição de armazenamento

protect from light

fluorescência

λex 551 nm; λem 567 nm (PKH26 dye)

método de detecção

fluorometric

Condições de expedição

ambient

temperatura de armazenamento

room temp

Categorias relacionadas

Descrição geral

O PKH26 é um corante vermelho-fluorescente. Este corante de membrana lipofílico é amplamente utilizado para determinar a proliferação celular em células heterogêneas por citometria de fluxo. [1]

Aplicação

Este kit destina-se à marcação em geral da membrana celular. Ele foi caracterizado em uma série de sistemas-modelo e foi considerado útil para a marcação de células in vitro, em estudos de proliferação in vitro e no rastreamento de células in vivo de longo prazo. A meia-vida para a eluição de PKH26 de hemácias de coelhos marcadas é superior a 100 dias. Essa maior estabilidade é favorável para estudos in vivo de longo prazo.
O kit ligante de células com vermelho fluorescente PKH26 destina-se à marcação geral da membrana celular. Ele foi caracterizado em uma série de sistemas-modelo e foi considerado útil para a marcação de células in vitro em estudos de proliferação in vitro e no rastreamento de células in vivo de longo prazo.[2][3] A meia-vida para a eluição de PKH26 de hemácias de coelhos marcadas é superior a 100 dias. Essa maior estabilidade é favorável para estudos in vivo de longo prazo.

Ligação

Para obter mais detalhes técnicos sobre os corantes fluorescentes ligantes de células PKH e CellVue®, incluindo uma extensa bibliografia, visite aqui.

Informações legais

CellVue is a registered trademark of Phanos Technologies

Somente componentes do kit

Nº do produto
Descrição
  • Diluent C 6 x 10
  • PKH26 Cell Linker in ethanol .5 mL

produto relacionado

Nº do produto
Descrição
Preços

Pictogramas

FlameExclamation mark
GHS02,GHS07

Palavra indicadora

Danger

Frases de perigo

H225,H319

Classificações de perigo

Eye Irrit. 2 - Flam. Liq. 2

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

57.2 °F - closed cup

Ponto de fulgor (°C)

14.0 °C - closed cup

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Certificados de análise (COA)

Lot/Batch Number
MKCV7397
MKCV4102
MKCV7789
MKCR6075
MKCQ6477

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Questions

1–10 of 10 Questions  

  1. If we want to use the PKH to label the membrane of fibroblast, and then co-culture them with tumor cells in order to interact, ¿ Will PKH transfer from fibroblast to tumor cells?

    1 answer

    1. PKH dyes are routinely used in co-culture situations. Because staining is nearly instantaneous, rapid and homogeneous dispersion of cells in dye solution is essential. There should be no excess or unincorporated dye in the medium prior to mixing or co-culturing the cell lines. Free dye will be taken up by all cells. See the links below to the product protocol as well as two additional co-culture studies using PKH dyes:

      Protocol
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf

      Co-culture in scaffolds
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155425/

      Co-culture, mixed monolayer, and on Transwell
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5168417/

      Helpful?

  2. When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?

    1 answer

    1. Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.

      Helpful?

  3. Product PKH26GL, PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, is a red dye.  Do you offer a kit that stains the cells green?

    1 answer

    1. We offer Product numbers:PKH2GL Both of these kits stain the cells with a dye that emits in the green fluorescent range.

      Helpful?

  4. Are cells lost during the PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling staining process?

    1 answer

    1. Over-labeling of the cells will result in loss of membrane integrity and reduced cell recovery.  Methods for improving cell viability can be found on the troubleshooting guide.

      Helpful?

  5. Does the dye leak from the cells after labeling when using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling?

    1 answer

    1. In general, slow loss of fluorescence has been observed with PKH1 and PKH2 in in vivo studies and it is likely that PKH67 may also slowly lose fluorescence. Leakage (or loss of staining intensity) may also occur due to cell-to-cell transfer of the dye. The most common cause of cell-to-cell dye transfer is inadequate washing of the cells. Wash cells 3-5 times after labeling and transfer samples to new tubes between washes.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. How many cells can be stained with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Products PKH26GL and MINI26?

    1 answer

    1. For Product PKH26GL, when using the concentrations indicated in the product bulletin, you can stain 30 samples (1x107 cells/sample).The kit provides 500 ul of PKH26 dye solution.  You use 4 ul per sample.  There is enough dye in the kit to stain 125 samples.The kit provides 60 mls of Diluent C.  You use 2 mls per sample (1 ml for dilution of the dye, 1 ml for dilution of the cells).  There is enough diluent for 30 samples.You can purchase additional diluent as Product CGLDILIf you need less dye, you can purchase Product MINI26 which contains 100 ul of dye and 10 mls of Diluent C.  This kit contains enough dye to stain 25 samples and enough diluent to stain 5 samples.

      Helpful?

  8. What is the difference between PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling Products PKH26GL and PKH26PCL?

    1 answer

    1. The general labeling kit (Product No. PKH26GL) will label the membrane of the cells.  For phagocytic cells, Product No. PKH26PCL is used.  This kit utilizes dye aggregates for specific labeling of phagocytic cells in the presence of other cell types.

      Helpful?

  9. What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?

    1 answer

    1. A protocol for visualization of tissue sections can be found in the technical bulletin.  For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells.  If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.

      Helpful?

  10. Will Product PKH26GL, PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling Products, stain dead cells?

    1 answer

    1. As long as the cell has an intact membrane, the PKH26 dye can label the cell.

      Helpful?

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