PMEF-CFX

EmbryoMax^® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Irradiated, Passage 3

• Sinônimo(s): CF-1 MEFs, CF-1 PMEFs, Irradiated MEFs, MEF Feeder Cells, MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells, Irradiated MEFS, Mouse Feeder Cells
• Código UNSPSC: 41106509
• eCl@ss: 32011203
• NACRES: NA.81

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• fabricante/nome comercial: Specialty Media; EmbryoMax^®
• técnica(s): cell culture | stem cell: suitable
• entrada: sample type primary embryotic fibroblasts (PMEFs); sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s); sample type: mouse embryonic stem cell(s)
• Condições de expedição: liquid nitrogen

Descrição

Descrição geral

The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

These PMEF cells were isolated from CF-1 mouse embryos at day 13 and mitotically inactivated by treatment with irradiation. The cells can be directly plated onto gelatin coated (Cat. No. ES-006-B) culture vessels. These cells are from passage 3. 5-6 x 10⁶ cells will produce the appropriate density feeder layer on the following tissue culture vessels:

• 6 x 60mm dishes
• 2 x T75 flasks
• 2 x 6 well plates

Refer to Murine Embryonic Stem Cell Culture Manual for complete culture protocols and media formulations for PMEF cells.

Descrição de linhagem celular

Primary Mouse Embryo Fibroblasts

Aplicação

Research Category
Stem Cell Research

Embalagem

5-6x10⁶ ea

Armazenamento e estabilidade

Vials should be stored in the vapor phase of liquid nitrogen.

Informações legais

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable