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AB758

Sigma-Aldrich

Anti-Collagen Type I Antibody

Chemicon®, from goat

Sinônimo(s):

Anti-CAFYD, Anti-EDSARTH1, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

goat

Nível de qualidade

forma do anticorpo

affinity purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

human, bovine

fabricante/nome comercial

Chemicon®

técnica(s)

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

adequação

not suitable for immunohistochemistry (Paraffin)

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... COL1A1(1277)

Especificidade

Recognizes Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Imunogênio

Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.

Aplicação

Anti-Collagen Type I Antibody is an antibody against Collagen Type I for use in DB, ELISA, IC, IH & WB.
Dot and slot blotting: 1:100-1:500

ELISA: 1:50-1:100

Indirect immunohistochemistry (frozen sections only): 1:10-1:20

Indirect immunocytochemistry: 1:10-1:20

Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.

forma física

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose 4B.. Liquid in 0.5 mL of 100 mM borate buffered saline, pH 8.0, no preservatives.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

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Pictogramas

Health hazard

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Repr. 1B

Código de classe de armazenamento

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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