524627

Phosphatase Inhibitor Cocktail III

liquid, for the inhibition of serine/threonine and protein tyrosine phosphatases

• Sinônimo(s): Phosphatase Inhibitor Mix (Set III), Phosphatase inhibitor
• Código UNSPSC: 12352200
• NACRES: NA.77

Propriedades

• Nome do produto: Phosphatase Inhibitor Cocktail Set III, Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.
• Formulário: liquid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• solubilidade: water: soluble
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.

Aplicação

Phosphatase Inhibitor Cocktail Set III has been used:

• as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting[1]
• to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis[2]
• as a component in cell lysis buffer to extract total proteins for western blotting[3]

Ações bioquímicas/fisiológicas

Cell permeable: no

Primary Target
Serine/threonine and protein tyrosine phosphatases

Product does not compete with ATP.

Reversible: no

Características e benefícios

• Ready-to-use formulations for convenient use
• High-quality products for consistent and reproducible performance,
• Flexible options with a range of cocktails targeting different categories of phosphatases

Componentes

Each vial contains 1 ml of aqueous solution with the following components:

• 50 mM Sodium Fluoride
• 10 mM ß-Glycerophosphate (Cat. No. 35675)
• 10 mM Sodium Pyrophosphate Decahydrate
• 1 mM Sodium Orthovanadate

Advertência

Toxicity: Toxic (F)

forma física

In H₂O.

Reconstituição

For long-term storage, aliquot and freeze (-20°C). Dilute 1:5 just prior to use.

Outras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable