524625

Phosphatase Inhibitor Cocktail II

liquid, for the inhibition of acid phosphatases, alkaline phosphatases and protein tyrosine phosphatases (PTP's). Suitable for use with cell lysates and tissue extracts.

• Sinônimo(s): Phosphatase Inhibitor, Phosphatase Inhibitor Solution (Set II)
• Código UNSPSC: 12352200
• NACRES: NA.77

Propriedades

• Nome do produto: Phosphatase Inhibitor Cocktail Set II, A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts.
• Nível de qualidade: 100
• Formulário: liquid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• solubilidade: water: soluble
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents.

Aplicação

Phosphatase Inhibitor Cocktail Set II has been used:

• in triple detergent buffer to lyse cultured cells to extract total protein for western blots[1]
• as a component of lysis buffer to lyse tissue samples or cultured rat ventricular cardiomyocytes (VCMs) for western blot analysis of cardiac mitochondrial proteins[2]
• as a component of lysis buffer to lyse cultured rat VCMs infected with Adv-COX7RP and Adv-shRNA COX7RP for immunoprecipitation of ryanodine receptors type 2 (RyR2) [2]
• as a component in ice-cold RIPA buffer to homogenize/lyse retina tissues for protein extraction and western blotting[3]

Ações bioquímicas/fisiológicas

Protein phosphorylation and dephosphorylation play essential roles in signal transduction processes that regulate various cellular functions including cell division, proliferation, and apoptosis. Protein kinases transfer phosphate groups to specific serine, threonine, or tyrosine residues, leading to a conformational change in the protein and affecting its interaction with ligands. Protein phosphatases reverse this process by removing the phosphate group and restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain the phosphorylation state of the proteins intact.

Características e benefícios

• Flexible: compatible with cell lysates and tissue extracts, including samples containing detergents.
• Economical: ready-to-use format minimizes wastage.
• Effective: one ml is adequate for approximately 100 ml extraction buffer.
• Complete: contains five phosphatase inhibitors targeting acid and alkaline phosphatases and protein tyrosine phosphatases (PTPs).

Componentes

Provided as a set of five 1 ml vials containing the following components:

• Imidazole (200 mM)
• Sodium Fluoride (100 mM)
• Sodium Molybdate (115 mM)
• Sodium Orthovanadate (100 mM)
• Sodium Tartrate Dihydrate (400 mM)      

Advertência

Toxicity: Standard Handling (A)

Outras notas

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS08,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H315,H319,H360D
• Declarações de precaução: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classificações de perigo: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Código de classe de armazenamento: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable