325885

c-MET, GST-Fusion, Human, Recombinant, S. frugiperda

c-MET, GST-Fusion, Human, Recombinant (aa 956-1390) fused at the N-terminus to a GST-His6-thrombin cleavage site and expressed in S. frugiperda. c-Met is a receptor tyrosine kinase activated by HGF.

• Sinônimo(s): Hepatocyte growth factor receptor tyrosine kinase
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• recombinante: expressed in baculovirus infected Sf21 cells
• Nível de qualidade: 100
• Formulário: liquid
• atividade específica: ≥30 units/mg protein
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• Condições de expedição: wet ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Recombinant, human MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Recombinant, human c-MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). c-MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala, Glu, Lys, Tyr)_6:2:5:1 per min at 30°C, using variable concentrations of ATP (0.1-1.48 µM).

forma física

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.

Reconstituição

Following initial thaw, aliquot and freeze (-70°C).

Outras notas

Palka, H.L., et al. 2003. J. Biol. Chem.278, 5278.
Vadnais, J., et al. 2002. J. Biol. Chem.277, 48342.
Crostella, L., et al. 2001. Oncogene20, 3735.
Guiton, M., et al. 1994. J. Biol. Chem269, 30370.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1