234109-M

Colcemid

Cell synchronization agent.

• Sinônimo(s): Colcemid, Demecolcine
• Fórmula empírica (Notação de Hill): C₂₁H₂₅NO₅
• Número CAS: 477-30-5
• Peso molecular: 371.43
• Número MDL: MFCD00075459
• Código UNSPSC: 12352200

Propriedades

• Ensaio: ≥98% (HPLC)
• forma: crystalline powder
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• solubilidade: chloroform: 10 mg/mL; ethanol: soluble
• temperatura de armazenamento: 10-30°C
• InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3
• chave InChI: NNJPGOLRFBJNIW-UHFFFAOYSA-N

Descrição

Descrição geral

Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.

Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate the spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.

Ações bioquímicas/fisiológicas

Primary Target
Depolymerizes microtubules and limits microtubule formation.

Advertência

Toxicity: Toxic & Carcinogenic / Teratogenic (G)

Reconstituição

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 6 months at -20°C.

Outras notas

Urbani, L., et al. 1995. Exp. Cell Res. 219, 159.
Kanda, R., et al. 1994. J. Radiat. Res. 35, 41.
Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373.
Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res.198, 214.
Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res.31, 494.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany