T1895

Timidina

powder, BioReagent, suitable for cell culture

• Sinônimo(s): 1-(2-Desoxi-β-D-ribofuranosil)-5-metiluracil, 1-(2-Desoxi-β-D-ribofuranosil)timina, 2′-Desoxitimidina, Timina desoxirribosídeo, dT
• Fórmula empírica (Notação de Hill): C₁₀H₁₄N₂O₅
• Número CAS: 50-89-5
• Peso molecular: 242.23
• Beilstein: 89285
• Número CE: 200-070-4
• Número MDL: MFCD00006537
• Código UNSPSC: 12352207
• ID de substância PubChem: 24899996
• NACRES: NA.75

Propriedades

• fonte biológica: synthetic (organic)
• Nível de qualidade: 300
• linha de produto: BioReagent
• Ensaio: ≥99%
• Formulário: powder
• técnica(s): cell culture | mammalian: suitable
• pf: 186-188 °C (lit.)
• solubilidade: water: 50 mg/mL, clear, colorless
• cadeia de caracteres SMILES: CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O
• InChI: 1S/C10H14N2O5/c1-5-3-12(10(16)11-9(5)15)8-2-6(14)7(4-13)17-8/h3,6-8,13-14H,2,4H2,1H3,(H,11,15,16)/t6-,7+,8+/m0/s1
• chave InChI: IQFYYKKMVGJFEH-XLPZGREQSA-N
• Informações sobre genes: human ... DTYMK(1841)

Descrição

Descrição geral

Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine.

Aplicação

Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used:

• as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts
• as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies
• to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells

Ações bioquímicas/fisiológicas

Thymidine is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)