Lot-specific information can be found on the certificate of analysis (COA). For example, for lot #: 3952706, the specific activity is 3.0 U/mgP, and the pack size is 100 milliunits, which is equivalent to 0.1 units. Therefore, for this specific lot, there is 0.033 mg of protein. In general, for this material, each mg is about 2 to 4 units, so 100 milliunits or 0.1 unit is equivalent to about 0.025 to 0.05 mg amount of protein.
219373
Cathepsin G, Human Neutrophil
Cathepsin G, Human Neutrophil, CAS 107200-92-0, is a purified native cathepsin G. Acts as a potent agonist of human platelet activation leading to their aggregation.
Sinônimo(s):
Cathepsin G, Human Neutrophil
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About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.77
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
human neutrophils
Nível de qualidade
descrição
Merck USA index - 14, 1905
Ensaio
≥95% (SDS-PAGE)
Formulário
lyophilized solid (Salt-free)
atividade específica
≥2 units/mg protein
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
técnica(s)
inhibition assay: suitable
adequação
suitable for molecular biology
aplicação(ões)
life science and biopharma
Condições de expedição
ambient
temperatura de armazenamento
−20°C
Informações sobre genes
human ... CTSG(1511)
Descrição geral
Research area: Cell Signaling
Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.[1]
Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.[1]
Aplicação
Ações bioquímicas/fisiológicas
This protease cleaves the triple-helix structure of type I collagen in a restricted manner and degrades nonhelical regions of type I and II collagens, fibronectin, aggrecan, and elastin. Additionally, cathepsin G activates ProMMP-3 and -8.[1] Cathepsin G serves as a chemoattractant for human phagocytes and enhances the random motility of T cells. Additionally, it initiates chemotaxis, activates ERK1/2 and p38 MAPK, and facilitates the translocation of PKCζ to the cell membrane, without inducing a detectable calcium flux.[4] Other biological functions of cathepsin G include: ECM components and plasma proteins degradation, bactericidal actions, cleavage of inflammatory mediators, conversion of angiotensin I to angiotensin II, platelet activation and induction of airway submucosal gland secretion.[5]
Advertência
Toxicity: Standard Handling (A)
Definição da unidade
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-AAPF-pNA per min at 25°C, pH 7.5.
Nota de preparo
Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Reconstitute in 150 mM NaCl, 50 mM sodium acetate buffer, pH 5.5.
Reconstitute in 150 mM NaCl, 50 mM sodium acetate buffer, pH 5.5.
Reconstituição
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -70°C.
Reconstitute in 50 mM NaOAc and 150 mM NaCl, pH 5.5.
Outras notas
Glusa, E., and Adam C. 2001. Por. J. Pharmacol.133, 422.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Polymorphonuclear neutrophils form a primary line of defense against bacterial infections using complementary oxidative and non-oxidative pathways to destroy phagocytized pathogens. The three serine proteases elastase, proteinase 3 and cathepsin G, are major components of the neutrophil primary granules that
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Although the Streptococcus pneumoniae polysaccharide capsule is an important virulence factor, ~ 15% of carriage isolates are nonencapsulated. Nonencapsulated S. pneumoniae are a cause of mucosal infections. Recent studies have shown that neutrophils kill S. pneumoniae predominately through neutrophil proteases, such as elastase
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Could you please provide the item number for R: 219373? I recently purchased Human Neutrophil Cathepsin G (219373) with a concentration of 100MIU. I'm experiencing difficulty converting it to µg/ml for the required dilution. Can you assist me with this? Thank you.
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