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D-073

Supelco

5α-Dihydrotestosterone (DHT) solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Sinônimo(s):

5α-Dihydrotestosterone solution, 5-alpha-Dihydrotestosterone, DHT, Dihydrotestosterone, 5α−DHT solution

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About This Item

Fórmula empírica (Notação de Hill):
C19H30O2
Número CAS:
521-18-6
Peso molecular:
290.44
Número MDL:
MFCD00003667
Código UNSPSC:
41116107
ID de substância PubChem:
329798632
NACRES:
NA.24
Preço e disponibilidade não estão disponíveis no momento.

grau

certified reference material

Nível de qualidade

300

Formulário

liquid

Características

Snap-N-Spike®/Snap-N-Shoot®

embalagem

ampule of 1 mL

fabricante/nome comercial

Cerilliant®

concentração

1.0 mg/mL in methanol

técnica(s)

gas chromatography (GC): suitable
liquid chromatography (LC): suitable

aplicação(ões)

clinical testing
clinical testing

Formato

single component solution

temperatura de armazenamento

−70°C

cadeia de caracteres SMILES

C[C@]12CC[C@H]3[C@@H](CC[C@H]4CC(=O)CC[C@]34C)[C@@H]1CC[C@@H]2O

InChI

1S/C19H30O2/c1-18-9-7-13(20)11-12(18)3-4-14-15-5-6-17(21)19(15,2)10-8-16(14)18/h12,14-17,21H,3-11H2,1-2H3/t12-,14-,15-,16-,17-,18-,19-/m0/s1

chave InChI

NVKAWKQGWWIWPM-ABEVXSGRSA-N

Categorias relacionadas

Descrição geral

This Certified Spiking Solution® is suitable for GC/MS and LC/MS applications from clinical and diagnostic testing and endocrinology to sports testing.

5α-Dihydrotestosterone (DHT) is a steroid hormone and metabolite of the natural androgen, testosterone, formed by the action of NADPH-dependent enzyme 5α-reductase. It has been implicated in a broad range of diseases from male pattern baldness to benign prostatic hyperplasia and prostate cancer. As an anabolic steroid, DHT has also been used as a performance-enhancing drug to promote muscle growth.

Aplicação

The Certified Reference Material (CRM) in solution can also be used as follows:
  • Simultaneous analysis of testosterone and 5α-dihydrotestosterone using a benchtop gas chromatograph-mass spectrophotometer (GC-MS) in human serum samples
  • Liquid chromatography-electrospray ionization-tandem mass spectrometric (LC/ESI-MS/MS) determination of testosterone and 5α-dihydrotestosterone following their proton affinitive derivatization using picolinic acid and its analogs
  • Determination of testosterone and 5α-dihydrotestosterone in plasma and testis samples of fathead minnow by a gas chromatography-tandem mass spectrometric (GC-MS/MS) method
  • Multi-residue screening of epitestosterone, testosterone, 5α-dihydrotestosterone, dehydroepiandrosterone, androsterone, and etiocholanolone in urine samples by high-resolution full scan-electrospray ionization-liquid chromatography (HR-FS-ESI-LC) combined with quadrupole orbitrap mass spectrometer (Q/Orbitrap MS) following requirements of World Antidoping Agency (WADA)
  • Quantitative analysis of nine hydroxy-androgens and their conjugates by a stable isotope dilution liquid chromatography-electrospray ionization-tandem mass spectrometry (SID-LC-ESI-MS/MS) based method following their derivatization using picolinic acid

Características e benefícios

  • Fully characterized under ISO/IEC 17025 and ISO 17034 accreditation
  • Accompanied with a comprehensive Certificate of Analysis (CoA) with data on stability, homogeneity, accuracy of concentration, uncertainty, and traceability
  • Rigorously tested through real-time stability studies to ensure accuracy and shelf life
  • Gravimetrically prepared using qualified precision balances to ensure minimal uncertainty
  • Flame sealed under argon into ampoules for long-term shelf life
  • Offered in a convenient, DEA-exempt format to improve laboratory efficiency

Informações legais

CERILLIANT is a registered trademark of Merck KGaA, Darmstadt, Germany
CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation
Snap-N-Shoot is a registered trademark of Cerilliant Corporation
Snap-N-Spike is a registered trademark of Merck KGaA, Darmstadt, Germany

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Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Órgãos-alvo

Eyes,Central nervous system

Código de classe de armazenamento

3 - Flammable liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

49.5 °F - closed cup

Ponto de fulgor (°C)

9.7 °C - closed cup

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Certificados de análise (COA)

Lot/Batch Number

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Julia Johansson et al.
PloS one, 8(1), e54357-e54357 (2013-01-26)
In rats with dihydrotestosterone (DHT)-induced polycystic ovary syndrome (PCOS), repeated low-frequency electrical stimulation of acupuncture needles restores whole-body insulin sensitivity measured by euglycemic hyperinsulinemic clamp. We hypothesized that electrical stimulation causing muscle contractions and manual stimulation causing needle sensation have
Angel On Kei Chan et al.
Clinical chemistry, 59(5), 798-806 (2013-03-21)
5α-Reductase 2 deficiency (5ARD) is a known cause of 46,XY disorders of sex development (DSD). Traditionally, the diagnosis relies on dihydrotestosterone (DHT) measurement, but the results are often equivocal, potentially leading to misdiagnosis. We reviewed alternative approaches for diagnosis of
Paula M Marchetti et al.
Annals of clinical biochemistry, 50(Pt 2), 95-107 (2013-02-23)
Dihydrotestosterone (DHT) is the most potent natural androgen in humans. There has been an increasing interest in this androgen and its role in the development of primary and secondary sexual characteristics as well as its potential roles in diseases ranging
Changmeng Cai et al.
The Journal of clinical investigation, 123(3), 1109-1122 (2013-02-22)
Fusion of the androgen receptor-regulated (AR-regulated) TMPRSS2 gene with ERG in prostate cancer (PCa) causes androgen-stimulated overexpression of ERG, an ETS transcription factor, but critical downstream effectors of ERG-mediating PCa development remain to be established. Expression of the SOX9 transcription
Jee In Kim et al.
American journal of physiology. Renal physiology, 305(1), F61-F70 (2013-05-10)
Male gender and the male hormone testosterone increase susceptibility to kidney ischemia and reperfusion (I/R) injury, which is associated with inflammatory responses. Possible involvement of histone deacetylase (HDAC) in inflammatory responses has been suggested. We investigated the gender-specific role of
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Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Does this DHT cross react between humans and mice? cat #: D-073-1ML

    1 answer

    1. DHT exhibits cross-reactivity between humans and mice, but biological effects and responses may differ due to species-specific differences. This product is an analytical standard and is not intended for animal studies.

      Helpful?

  4. Is this suitable for cell culture?

    1 answer

    1. This product is prepared in methanol, which would be toxic to cells. Currently, there is no cell culture tested product option available. However, product A8380, the powder form, has been used successfully in cell culture applications. Please see the link below to review this product. Note the Peer Reviewed Papers section lists a number of applicable publications.
      https://www.sigmaaldrich.com/product/sigma/a8380

      Helpful?

  5. hi, am I right in thinking that D-073 arrives in 1mL vials and they are to be stored long term at -70C. We only have -80C freezers, do you see that as a problem?

    1 answer

    1. The product COA (https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/fluka/pdf/cer/D-073_FE02172263.pdf) indicates on page 10 that it is okay to store this material for long term stability at -60C to -80C, so the indicated freezer/temperature should be fine.

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