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Documentos Principais

935409

Sigma-Aldrich

Sulfo-NHS-LC-LC-Biotin

≥95%

Sinônimo(s):

3-Pyrrolidinesulfonic acid, 1-[[6-[[6-[[5-(hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl)-1-oxopentyl]amino]-1-oxohexyl]amino]-1-oxohexyl]oxy]-2,5-dioxo-, monosodium salt, [3aS-(3aα,4β,6aα)]-[partial]-, EZ-Link Sulfo-NHS-LC-LC-Biotin, Hexanoic acid, 6-[[6-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]-1-oxohexyl]amino]-, 2,5-dioxo-3-sulfo-1-pyrrolidinyl ester, sodium salt (1:1), Sulfo-NHS-XX-Biotin

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About This Item

Fórmula empírica (Notação de Hill):
C26H40N5O10S2·Na
Número CAS:
Peso molecular:
669.74
Número MDL:
Código UNSPSC:
12352100
NACRES:
NA.21
Preço e disponibilidade não estão disponíveis no momento.

Nível de qualidade

Ensaio

≥95%

Formulário

solid

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

[Na].O=C1NC2CSC(CCCCC(=O)NCCCCCC(=O)NCCCCCC(=O)ON3C(=O)CC(C3=O)S(=O)(=O)O)C2N1

InChI

InChI=1S/C26H41N5O10S2.Na/c32-20(27-14-8-2-4-12-23(35)41-31-22(34)15-19(25(31)36)43(38,39)40)10-3-1-7-13-28-21(33)11-6-5-9-18-24-17(16-42-18)29-26(37)30-24;/h17-19,24H,1-16H2,(H,27,32)(H,28,33)(H2,29,30,37)(H,38,39,40);/t17-,18-,19?,24-;/m0./s1

chave InChI

RWNPTJCDHAXLGI-BJPAGVOZSA-N

Aplicação

Sulfo-NHS-LC-LC-biotin finds extensive applications in scientific research for the purpose of protein labeling and detection. Its utilization in protein purification allows for the isolation of biotinylated proteins from intricate mixtures. Moreover, it plays a vital role in protein-protein interaction studies, enabling investigations into the binding affinity and specificity of proteins. Additionally, it serves as a valuable tool in protein quantification, facilitating the measurement of specific protein levels within a given sample.

Características e benefícios

Sulfo-NHS-LC-LC-biotin is a frequently employed chemical compound in scientific investigations aimed at examining and characterizing proteins. This compound serves as a biotinylation agent, allowing for the labeling of proteins in diverse applications, including protein purification, studies on protein-protein interactions, and protein quantification. Sulfo-NHS-LC-LC-biotin works by reacting with the primary amines of proteins to form stable amide bonds. This results in the biotinylation of the protein, which allows for the detection and isolation of the protein using streptavidin or avidin conjugated to a detection system, such as a fluorescent or radioactive label.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Visite a Biblioteca de Documentos

D Castelletti et al.
Clinical and experimental immunology, 136(2), 365-372 (2004-04-17)
Hodgkin's lymphoma patients treated with an anti-CD25 Ricin toxin A-chain (RTA)-based Immunotoxin (RFT5.dgA) develop an immune response against the toxic moiety of the immunoconjugate. The anti-RTA antibody response of 15 patients showing different clinical features and receiving different total amounts
M Ichikawa et al.
Bioorganic & medicinal chemistry letters, 11(13), 1769-1773 (2001-06-27)
We have prepared several mechanism-based affinity-labeling agents for possible use in isolating N-acetylglucosaminidase, in which an N-acetylglucosamine is linked to an o-monofluoro- or difluoro-methyl phenoxy glycoside with or without a cleavable disulfide group in the tether to biotin.
A reagent for covalently attaching biotin to proteins via a cleavable connector arm.
C A Mouton et al.
Archives of biochemistry and biophysics, 218(1), 101-108 (1982-10-01)
Chemical probes of extended biological structures: synthesis and properties of the cleavable protein cross-linking reagent [35S]dithiobis(succinimidyl propionate).
A J Lomant et al.
Journal of molecular biology, 104(1), 243-261 (1976-06-14)

Questions

  1. Whats the protocol used for biotinylation using this reagent (935409)?

    1 answer
    1. The method of biotinylating is considered proprietary. However, a general reference regarding the biotinylation of proteins is listed in the 'PEER REVIEWED PAPERS' section of the product page:

      A reagent for covalently attaching biotin to proteins via a cleavable connector arm.
      C A Mouton et al.
      Archives of biochemistry and biophysics, 218(1), 101-108 (1982-10-01)

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