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Documentos Principais

200964

Sigma-Aldrich

Erythrosin B

Dye content ≥95 %

Sinônimo(s):

Solvent Red 140

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5 G
R$ 558,00
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R$ 1.910,00

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5 G
R$ 558,00
25 G
R$ 1.910,00

About This Item

Fórmula empírica (Notação de Hill):
C20H8I4O5
Número CAS:
Peso molecular:
835.89
Número do índice de cores:
45430:2
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.47

R$ 558,00


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Formulário

powder

Nível de qualidade

composição

Dye content, ≥95%

técnica(s)

microbe id | staining: suitable

cor

orange to red-orange

pf

303 °C (dec.) (lit.)

solubilidade

1 M NH4OH: 1 mg/mL
1 M NH4OH: soluble 1 mg/mL, orange to red

ε (coeficiente de extinção)

≥102000 at 526-534 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
≥13000 at 310-318 nm in 1 M NH4OH at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
≥32000 at 258-266 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

Oc1c(I)cc2c(Oc3c(I)c(O)c(I)cc3C24OC(=O)c5ccccc45)c1I

InChI

1S/C20H8I4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H

chave InChI

OALHHIHQOFIMEF-UHFFFAOYSA-N

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Aplicação

Erythrosin B has been used as a dye in fluorescence lifetime imaging.[1][2]

Ações bioquímicas/fisiológicas

Erythrosin B, also called as eosin B, is a xanthene dye.[3] It belongs to the family of fluorescein dyes. It plays a major role in inhibiting dopamine uptake and high affinity 3H-ouabain binding and ion transport in synaptosomes from rat caudate nucleus.[4][5]

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Bright monomeric red fluorescent protein with an extended
fluorescence lifetime
Merzlyak EM
Nature Methods, 4, 555-557 (2007)
Erythrosin B inhibits dopamine transport in rat caudate synaptosomes.
Lafferman JA and Silbergeld EK
Science, 205, :410-:412 (1979)
Erythrosin B is a specific inhibitor of high affinity 3H-ouabain binding and ion transport in rat brain
Silbergeld E K
Neuropharmacology, 20, 87-90 (1981)
Practical and reliable FRET/FLIM pair of fluorescent proteins.
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Because of the serious harm to animals and the environment associated with perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), a rapid, sensitive and low-cost method for detecting PFOS and PFOA is of great importance. In this paper, a novel sensing

Questions

1–2 of 2 Questions  
  1. How to prepare for Erythrosin B (200964) 0,5%?

    1 answer
    1. Erythrosin B solutions can be prepared in various solvents. The dye is commonly prepared in water, buffered salt solutions or in ethanol. The Biological Stain Commission dissolves the dye in clove oil for the testing that is performed for certification of biological dyes. The solvent chosen will depend upon the staining applications. Erythrosin B can be dissolved in water to ~11 percent and 5% in ethanol. Some application recommend mixing or vortexing the solutions before use

      Helpful?

  2. Are there instructions for using Erythrosin B for fluorescent microscopy?

    1 answer
    1. Erythrosin B and Eosin B are considered synonyms; however, there are differences in their CAS numbers and CI numbers. Although there are no specific instructions for the use of product 200964, instructions are available for another Erythrosin B. This product can be used in a light microscopy procedure called hematoxylin and eosin staining. Additionally, both Erythrosin B and Eosin B are commonly used in H&E staining procedures and may be suitable for fluorescence microscopy for viability staining. While there is no product available for a staining procedure for viability staining, multiple procedures are available on the internet that can be considered for use with product 200964.

      Helpful?

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