Erythrosin B solutions can be prepared in various solvents. The dye is commonly prepared in water, buffered salt solutions or in ethanol. The Biological Stain Commission dissolves the dye in clove oil for the testing that is performed for certification of biological dyes. The solvent chosen will depend upon the staining applications. Erythrosin B can be dissolved in water to ~11 percent and 5% in ethanol. Some application recommend mixing or vortexing the solutions before use
200964
Erythrosin B
Dye content ≥95 %
Sinônimo(s):
Solvent Red 140
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About This Item
Produtos recomendados
Formulário
powder
Nível de qualidade
composição
Dye content, ≥95%
técnica(s)
microbe id | staining: suitable
cor
orange to red-orange
pf
303 °C (dec.) (lit.)
solubilidade
1 M NH4OH: 1 mg/mL
1 M NH4OH: soluble 1 mg/mL, orange to red
ε (coeficiente de extinção)
≥102000 at 526-534 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
≥13000 at 310-318 nm in 1 M NH4OH at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
≥32000 at 258-266 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
aplicação(ões)
diagnostic assay manufacturing
hematology
histology
temperatura de armazenamento
room temp
cadeia de caracteres SMILES
Oc1c(I)cc2c(Oc3c(I)c(O)c(I)cc3C24OC(=O)c5ccccc45)c1I
InChI
1S/C20H8I4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H
chave InChI
OALHHIHQOFIMEF-UHFFFAOYSA-N
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Categorias relacionadas
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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fluorescence lifetime
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How to prepare for Erythrosin B (200964) 0,5%?
1 answer-
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Are there instructions for using Erythrosin B for fluorescent microscopy?
1 answer-
Erythrosin B and Eosin B are considered synonyms; however, there are differences in their CAS numbers and CI numbers. Although there are no specific instructions for the use of product 200964, instructions are available for another Erythrosin B. This product can be used in a light microscopy procedure called hematoxylin and eosin staining. Additionally, both Erythrosin B and Eosin B are commonly used in H&E staining procedures and may be suitable for fluorescence microscopy for viability staining. While there is no product available for a staining procedure for viability staining, multiple procedures are available on the internet that can be considered for use with product 200964.
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