Direkt zum Inhalt
Merck
  • Aspartic acid-based modified PLGA-PEG nanoparticles for bone targeting: in vitro and in vivo evaluation.

Aspartic acid-based modified PLGA-PEG nanoparticles for bone targeting: in vitro and in vivo evaluation.

Acta biomaterialia (2014-07-23)
Yin-Chih Fu, Tzu-Fun Fu, Hung-Jen Wang, Che-Wei Lin, Gang-Hui Lee, Shun-Cheng Wu, Chih-Kuang Wang
ZUSAMMENFASSUNG

Nanoparticles (NP) that target bone tissue were developed using PLGA-PEG (poly(lactic-co-glycolic acid)-polyethylene glycol) diblock copolymers and bone-targeting moieties based on aspartic acid, (Asp)(n(1,3)). These NP are expected to enable the transport of hydrophobic drugs. The molecular structures were examined by (1)H NMR or identified using mass spectrometry and Fourier transform infrared (FT-IR) spectra. The NP were prepared using the water miscible solvent displacement method, and their size characteristics were evaluated using transmission electron microscopy (TEM) and dynamic light scattering. The bone targeting potential of the NP was evaluated in vitro using hydroxyapatite affinity assays and in vivo using fluorescent imaging in zebrafish and rats. It was confirmed that the average particle size of the NP was <200 nm and that the dendritic Asp3 moiety of the PLGA-PEG-Asp3 NP exhibited the best apatite mineral binding ability. Preliminary findings in vivo bone affinity assays in zebrafish and rats indicated that the PLGA-PEG-ASP3 NP may display increased bone-targeting efficiency compared with other PLGA-PEG-based NP that lack a dendritic Asp3 moiety. These NP may act as a delivery system for hydrophobic drugs, warranting further evaluation of the treatment of bone disease.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Trifluoressigsäure, ReagentPlus®, 99%
Sigma-Aldrich
Trifluoressigsäure, suitable for HPLC, ≥99.0%
Sigma-Aldrich
Trifluoressigsäure, puriss. p.a., suitable for HPLC, ≥99.0% (GC)
Sigma-Aldrich
N-Hydroxysulfosuccinimid Natriumsalz, ≥98% (HPLC)
Sigma-Aldrich
Trifluoressigsäure, ≥99%, for protein sequencing
Sigma-Aldrich
Triethylamin, BioUltra, ≥99.5% (GC)
Sigma-Aldrich
L-Asparaginsäure, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
Tetramethylsilan, ACS reagent, NMR grade, ≥99.9%
Sigma-Aldrich
L-Asparaginsäure, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
Triethylamin, ≥99.5%
Sigma-Aldrich
Tetramethylsilan, ≥99.0% (GC)
Sigma-Aldrich
L-Asparaginsäure, BioXtra, ≥99% (HPLC)
Sigma-Aldrich
Triethylamin, ≥99%
Sigma-Aldrich
Triethylamin, for amino acid analysis, ≥99.5% (GC)
Sigma-Aldrich
L-Asparaginsäure, BioUltra, ≥99.5% (T)
Sigma-Aldrich
Triethylamin, ≥99.5%
Sigma-Aldrich
Triethylamin, for protein sequence analysis, ampule, ≥99.5% (GC)
SAFC
L-Asparaginsäure
Sigma-Aldrich
Calciumhydrid, 99.9% trace metals basis
Sigma-Aldrich
Calciumhydrid, reagent grade, 95% (gas-volumetric)
Sigma-Aldrich
Calciumhydrid, powder, 99.99% trace metals basis
Sigma-Aldrich
Triethylamin, puriss. p.a., ≥99.5% (GC)
Supelco
Trifluoressigsäure, analytical standard
Sigma-Aldrich
Tetramethylsilan, electronic grade, ≥99.99% trace metals basis
Sigma-Aldrich
Calciumhydrid, powder, 0-2 mm, reagent grade, ≥90% (gas-volumetric)
Supelco
Tetramethylsilan, analytical standard, for NMR spectroscopy, ACS reagent
Sigma-Aldrich
Calciumhydrid, purum p.a., ≥97.0% (gas-volumetric), powder
Supelco
Triethylamin, analytical standard
Supelco
L-Asparaginsäure, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
L-Asparaginsäure, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland