SRP5172

Catenin β, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): CTNNB, FLJ25606
• CAS-Nummer: 847964-78-7
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥70% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• Mol-Gew.: ~115 kDa
• Methode(n): activity assay: suitable
• Löslichkeit: water: soluble
• Eignung: suitable for molecular biology
• NCBI-Hinterlegungsnummer: NM_001904
• Anwendung(en): cell analysis
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... CTNNB1(1499)

Beschreibung

Allgemeine Beschreibung

Research area: Apoptosis. β-catenins are cytoplasmic proteins that are ubiquitously expressed. These proteins are associated with E-cadherin at cellular junctions.[1] CTNNB1 (catenin β-1) belongs to the armadillo family of proteins. This protein possesses a 130 amino acid N-terminal domain, 12 imperfect repeats of 42 amino acids termed arm repeats, and a 100 amino acid C-terminal domain. [2] The gene encoding β-catenin is localized on human chromosome 3p22.1.[3]

Anwendung

Catenin β, GST tagged human has been used:

• as a component of protein kinase buffer for in vitro kinase assay[4]
• in in vitro citrullination assay to characterize β-catenin citrullination[5]
• to perform differentiation factor screening[6]

Biochem./physiol. Wirkung

β-catenin interacts with T-cell factor and lymphoid enhancer-binding factor (TCF and LEF) transcription factors and is an essential member of the Wingless-Wnt signal transduction pathway.[1] The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and glycogen synthase kinase 3 β (GSK3β), form a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of βcatenin by the proteasome.[2] APC and the Siah-1 E3 ubiquitin-protein ligase-1 mediate a novel β-catenin degradation pathway linking p53 activation to cell cycle control. Activating mutations in the human β-catenin gene have been found in human colon cancer and melanomas.[7][8] The protein has a role in homeostasis and development.[2]

Physikalische Form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Angaben zur Herstellung

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable