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MITOISO1

Sigma-Aldrich

Mitochondrien-Isolierungs-Kit

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

Synonym(e):

Mitochondria Purification Kit

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise

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1 KIT
€ 1.190,00

€ 1.190,00

Voraussichtliches Versanddatum14. April 2025

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1 KIT
€ 1.190,00

About This Item

EG-Nummer:
232-650-8
UNSPSC-Code:
12352200
NACRES:
NA.32

€ 1.190,00

Voraussichtliches Versanddatum14. April 2025

Qualitätsniveau

200

Verwendung

sufficient for 10-20 g (animal tissue)
 sufficient for 50 assays (2 mL)

Haltbarkeit

2 yr

Methode(n)

fractionation: suitable

Versandbedingung

dry ice

Lagertemp.

−20°C

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Anwendung

Mitochondria Isolation Kit has been used:
  • for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity[1]
  • for the isolation of mitochondria from glomeruli[2] and heart[3]
  • for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential[4]

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

Leistungsmerkmale und Vorteile

  • Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
  • Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
  • Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
  • Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB
  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solidSDB

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Cytochrom-C-Oxidase-Assay-Kit, sufficient for 100 tests, soluble and membrane bound mitochondria

CS0720

Citrat-Synthase-Assay-Kit, 1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

MITOISO2

Mitochondrien-Isolierungs-Kit, sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

CS0760

Isolated Mitochondria Staining Kit, 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

MITOISO3

Yeast Mitochondria Isolation Kit, sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells

Piktogramme

Health hazardExclamation mark
GHS08,GHS07

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

10 - Combustible liquids

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000284757
0000284757
0000222575
0000222575
0000137714

Die passende Version wird nicht angezeigt?

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by preservation of mitochondrial membrane properties
Palzur E, et al.
Brain Research, 1221, 126-133 (2008)
Young-Kyoung Shin et al.
Cancer research, 65(8), 3162-3170 (2005-04-19)
5-Fluorouracil (5-FU) is widely used for treatment of advanced colorectal cancer. However, it is common for such patients to develop resistance to 5-FU, and this drug resistance becomes a critical problem for chemotherapy. The mechanisms underlying this resistance are largely
Mitochondrial dysfunction mediates aldosterone-induced podocyte damage: a therapeutic target of PPARgamma
Zhu C, et al.
The American Journal of Pathology, 178(5), 2020-2031 (2011)
DJ-1 protects the heart against ischemia-reperfusion injury by regulating mitochondrial fission
Shimizu Y, et al.
Journal of Molecular and Cellular Cardiology, 97, 56-66 (2016)
Emilyn U Alejandro et al.
Endocrinology, 151(2), 502-512 (2010-01-09)
Insulin enhances the proliferation and survival of pancreatic beta-cells, but its mechanisms remain unclear. We hypothesized that Raf-1, a kinase upstream of both ERK and Bad, might be a critical target of insulin in beta-cells. To test this hypothesis, we
Alle zugehörigen Dokumente anzeigen

Artikel

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Questions

1–8 of 8 Questions  

  1. Where is the step to get cytosol?

    1 answer

    1. The kit is intended for the isolation of intact mitochondria. It was not developed for cytoplasma collection. Nevertheless, reviewing the protocol, it can be assumed that the cytoplasmic fraction should be in the supernatant after the second centrifugation (point 8 of the Preparation of mitochondria from soft tissues protocol). Please keep in mind that this is not tested for suitability for cytoplasmic fraction.

      Please see product MIT1000 Mitochondria/Cytosol Fractionation Kit for efficient isolation of intact mitochondria from cultured cells. This kit can be used for tissue samples as long as the tissue is first homogenized into a single cell suspension. It may be helpful to use the same protocol that is recommended for cells following homogenization of the tissue into a single cell suspension in the supplied Isotonic Mitochondrial Buffer containing protease inhibitors, then follow the kits protocol for cells: https://www.sigmaaldrich.com/product/mm/mit1000.

      Helpful?

  2. Can Product MITOISO1, Mitochondria Isolation Kit, be used for isolation of mitochondria from cultured cells?

    1 answer

    1. It is possible to use this kit for cultured cells, however MITOISO2 is a kit that was specifically designed for this purpose.  This kit was originally designed for isolation from tissues.

      Helpful?

  3. When using Product MITOISO1, Mitochondria Isolation Kit, how long can I store my isolated mitochondria?

    1 answer

    1. If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.

      Helpful?

  4. When using Product MITOISO2, Mitochondria Isolation Kit, how can I store my isolated mitochondria?

    1 answer

    1. Please see below for information on how best to store your isolated mitochondria using the MITOISO2 kit:If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. Are the extraction buffers from Product MITOISO1, Mitochondria Isolation Kit, available separately?

    1 answer

    1. The extraction buffers supplied with this kit are only available with the kit.

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  7. Is the Product MITOISO1, Mitochondria Isolation Kit,  JC-1 stain available separately?

    1 answer

    1. Yes, while the component J4519 is not available separately, JC-1 stain is listed separately as Sigma Product No. T4069 or Product No. 40882.

      Helpful?

  8. How much tissue can I use with Product MITOISO1, Mitochondria Isolation Kit,?

    1 answer

    1. The procedures described in the bulletin are for small amounts of tissue samples (50-200 mg). Larger amounts of tissue can be purified with modifications listed in the technical bulletin.  We recommend a minimum of 50 mg.

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