MAK380

LDH-Cytotoxicity Assay Kit II

sufficient for 500 colorimetric tests

• UNSPSC-Code: 12161503

Eigenschaften

• Nachweisverfahren: colorimetric
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

The new LDH Cytotoxicity Assay Kit, MAK529, is now available! Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Lactate dehydrogenase (LDH) is a stable enzyme, present in all cell types, and rapidly released into the cell culture medium upon damage of the plasma membrane. LDH, therefore, is the most widely used marker in cytotoxicity study.

Leistungsmerkmale und Vorteile

The LDH-Cytotoxicity Assay Kit II:

• Is a non-radioactive assay.
• Simple one-step procedure.

Eignung

The kit is suitable for the determination of Lactate dehydrogenase (LDH) activity in cells.

Prinzip

The LDH Cytotoxicity Assay Kit II utilizes the advanced WST (Water Soluble Tetrazolium) reagent for a fast and more sensitive detection of LDH released from the damaged cells. The assay utilizes an enzymatic coupling reaction: LDH oxidizes lactate to generate NADH, which then reacts with WST to generate yellow color at A450 nm. The intensity of the generated color correlates directly with the cell number lysed.

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Danger
• H-Sätze: H314
• P-Sätze: P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405
• Gefahreneinstufungen: Eye Dam. 1 - Skin Corr. 1B
• Lagerklassenschlüssel: 8A - Combustible corrosive hazardous materials