LYSC9000

Lys-c (lysyl-endopeptidase), Active

from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), tag-free, solution

• Synonym(e): Lys-c (lysyl-endopeptidase), Active, API, Lysyl endopeptidase, Protease I
• EC-Nummer: 3.4.21.50 (BRENDA, IUBMB)
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Achromobacter lyticus
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli (206-473aa)
• Qualität: Proteomics Grade
• Form: ready-to-use solution
• Konzentration: 1 mg/mL
• Optimaler pH-Wert: 8.5
• pH-Bereich: 9.0-9.5(catalytic)
• UniProt-Hinterlegungsnummer: P15636
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

Spezifität

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

Anwendung

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

Verpackung

1mg/ml in Plastic

Angaben zur Herstellung

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

Lagerung und Haltbarkeit

Store product at -20°C for up to one year. Avoid freeze/thaw cycles.

Sonstige Hinweise

For R&D only.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable