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Mouse Monoclonal Antibody Isotyping Reagents

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

Synonym(e):

Mouse Antibody Isotyping Kit

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PackungsgrößeSKUVerfügbarkeitPreis
1 kit
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€ 391,00

Über diesen Artikel

UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
Storage temp.:
2-8°C
Shipped in:
wet ice

€ 391,00


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Quality Level

usage

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

shipped in

wet ice

storage temp.

2-8°C

General description

The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.

Application

Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.
Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA).[1][2] It may be used in Ouchterlony immunodiffusion assays.

Biochem/physiol Actions

ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.

Features and Benefits

  • May be used in a variety of assay formats
  • Suitable for all antibody forms
  • Determines all mouse IgG subclasses, IgA, and IgM

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Dieser Artikel
C7988A7554SAB4200669
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

usage

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

usage

-

usage

-

usage

-


Nur Kit-Komponenten

Produkt-Nr.
Beschreibung

  • goat antisera to mouse IgA .2 mL

  • goat antisera to mouse IgG3 .2 mL

  • goat antisera to mouse IgG1 .2 mL

  • goat antisera to mouse IgG2a .2 mL

  • goat antisera to mouse IgG2b .2 mL

  • goat antisera to mouse IgM .2 mL

Lagerklasse

10 - Combustible liquids



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Protokolle

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Verwandter Inhalt


Jorma Hinkula et al.
Vaccines, 7(3) (2019-07-19)
Background: Vaccination is commonly used to prevent and control influenza infection in humans. However, improvements in the ease of delivery and strength of immunogenicity could markedly improve herd immunity. The aim of this pre-clinical study is to test the potential
Preparation and Characterization of a Monoclonal Antibody with High Affinity for Soluble A β Oligomers
Ying,Z et al.
Hybridoma, 28(5) (2009)
Masanori Onda et al.
Journal of immunology (Baltimore, Md. : 1950), 177(12), 8822-8834 (2006-12-05)
Recombinant immunotoxins composed of an Ab Fv fragment joined to a truncated portion of Pseudomonas exotoxin A (termed PE38) have been evaluated in clinical trials for the treatment of various human cancers. Immunotoxin therapy is very effective in hairy cell



Global Trade Item Number

SKUGTIN
ISO2-1KT04061833866184

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