GE17-5079-02

HiTrap^® rProtein A Fast Flow

Cytiva 17-5079-02, pack of 2 × 1 mL

• UNSPSC-Code: 23151816
• NACRES: NA.56

Eigenschaften

• ligand: recombinant protein A (E. coli)
• Verpackung: pack of 2 × 1 mL
• Hersteller/Markenname: Cytiva 17-5079-02
• Lagerbedingungen: (20% Ehtanol); (20% Ethanol)
• Parameter: <4 mL/min flow rate; 42 psi
• Bettgröße: 7 mm × 25 mm
• Bettvolumen: 1 mL
• Säule ID: 7 mm
• Matrix: 4% cross-linked agarose
• Partikelgröße: 60-165 μm
• Durchschnittlicher Durchmesser: 90 μm (d50v)
• cleaning: 2-11
• Arbeitsbereich: 3-10
• Kapazität: ~50 mg binding capacity (human lgG/ml medium)
• Eignung: suitable for bioprocess medium
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.

HiTrap^® rProtein A FF columns are prepacked with rProtein A Sepharose^™ Fast FLow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Anwendung

The recombinant Protein A ligand (produced in E. coli) is coupled to Sepharose^™ Fast FLow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

Leistungsmerkmale und Vorteile

• Quick, convenient preparative purification of monoclonal antibodies.
• Well-established Protein A-based medium used in many approved MAb processes.
• Recombinant Protein A exhibits similar Fc region specificity to that of native Protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA^® design.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Lagerklassenschlüssel: 3 - Flammable liquids