CRE BAG2

95082219, mouse unknown/unspecified, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: CRE BAG2, 95082219
• Biologische Quelle: mouse unknown/unspecified
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse NIH 3T3 fibroblast, Moloney murine leukaemia virus transfected

Beschreibung der Zelllinie

CRE BAG2 has been established from the NIH 3T3 fibroblast cell line. Cells were transfected with Moloney murine leukaemia virus-derived proviral genomes carrying complementary mutations in the gag-pol or env regions. CRE BAG2 produces BAG ( a β-gal-transducing vector) and can be used for the package of vectors derived from murine leukaemia viruses. The production of recombinant replication-competent helper viruses has never been reported.

Anwendung

Virus-packaging assays, cell-lineage marking systems

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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