YTH 906.9HL

11060809

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Rat x Rat Hybridoma
• ImmunoDon: DA rat spleen
• Myelom: Y3.AG.1.2.3
• Reaktivität: Human IL2 receptor alpha (CD25)
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgG2b
• Versandbedingung: dry ice

Beschreibung

Ursprung der Zelllinie

Monoclonal antibody, mouse CD4 epitope A

Beschreibung der Zelllinie

Rat hybridoma producing antibody against human CD25. The designation HL indicates that it has bselected for loss of the original Y3 myeloma derived (non-specific) light chain. Cambridge Enterprise Reference number AB61:www.enterprise.cam.ac.uk.

Immunogen

Activated human T cells

Nährmedium

IDMEM (I3390) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin (T4424) or mechanical agitation maybe required to dislodge them from the plastic.

Subkultur-Routine

Maintain cultures between 3-9 x 10⁵ cells/ml. 5% CO₂; 37 °C. Saturation density 9 x 10⁵/ml.

Sonstige Hinweise

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Haftungsausschluss

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.