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A6017

Sigma-Aldrich

6-Aminohexyl-Agarose

saline suspension

Synonym(e):

ω-Aminohexyl-Agarose, 1,6-Diaminohexan-Agarose (N-gebunden)

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10 ML
€ 155,00
50 ML
€ 586,00
100 ML
€ 952,00

€ 155,00


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Größe auswählen

Ansicht ändern
10 ML
€ 155,00
50 ML
€ 586,00
100 ML
€ 952,00

About This Item

CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
23151817
NACRES:
NA.56

€ 155,00


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Biologische Quelle

plant

Form

saline suspension

Kennzeichnungsgrad

≥5 μmol per mL

Methode(n)

affinity chromatography: suitable

Matrix

cross-linked 4% beaded agarose

Matrixaktivierung

cyanogen bromide

Matrixanbindung

amino of 1,6-diaminohexane

Matrix-Spacer

1 atom

Kapazität

≥5 mg/mL binding capacity (bovine serum albumin)

Eignung

suitable for chromatography

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Hydrophobic ligands can serve as potentselective adsorbents. Both ω-aminoalkyl and alkyl agaroses can interact with the hydrophobic regions found in various proteins. The ω-aminoalkyl agarose product offered here consists of activated agarose with 1,6-diaminohexane covalently attached to one of its amine groups.

Anwendung

ω-Aminohexyl–Agarose has been used in the conjugation of fatty acids with chain length from two carbons to 20 for fatty acyl beads pull-down experiments.
ω-aminohexyl–agarose has been used for coupling of serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 for isolation of anti-SbAg antibodies using protein chromatography.[1]

Biochem./physiol. Wirkung

ω-aminoalkyl-agaroses are used in chromatography. In these, the protein is retained by lipophilic association between the hydrocarbon side chains on the agarose and hydrophobic pockets in the protein.[2]

Physikalische Form

Suspension in 0.5 M NaCl, enthält 0.02% Thiomersal

Sonstige Hinweise

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Piktogramme

Corrosion

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Eye Dam. 1 - Skin Irrit. 2

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Die Dokumentenbibliothek aufrufen

Y Ruttyn et al.
Journal of chromatography, 491(2), 299-308 (1989-07-21)
Human plasma was chromatographed under different experimental conditions on aminohexyl Sepharose. A strong retention of factor VIII/vWf and of factors VII, II, IX and X was observed. A satisfactory stabilization of eluted factor VIII clotting activity was obtained after addition
D Couchie et al.
The Biochemical journal, 199(2), 441-446 (1981-11-01)
Chromatography on hexyl-agarose resolved a partially purified cyclic GMP-activated phosphodiesterase from rat liver into two peaks of activity: the first was eluted with 0.5 M-KCl and was cyclic AMP-specific. The second was tightly bound to hexyl-agarose and was not eluted
Julio Blanco et al.
Acta crystallographica. Section D, Biological crystallography, 58(Pt 2), 352-354 (2002-01-25)
Aspartokinase III catalyzes the commitment step in the aspartate metabolism pathway, the phosphorylation of aspartic acid. The Escherichia coli enzyme has been crystallized in the presence of its natural substrate (aspartic acid) and Mg-ADP and diffraction data has been collected
Rapid isolation of microbial milk-clotting enzymes by N-acetyl (or N-isobutyryl)-pepstatin--Aminohexylagarose.
H Kobayashi et al.
Analytical biochemistry, 122(2), 308-312 (1982-05-15)
Hydrophobic chromatography: use for purification of glycogen synthetase.
Shaltiel S and Er-El Z
Proceedings of the National Academy of Sciences of the USA, 70, 778-781 (1973)

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