Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Wichtige Dokumente
43816
DL-Dithiothreitol -Lösung
BioUltra, for molecular biology, ~1 M in H2O
Synonym(e):
Clelands Reagenz, (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, (±)-threo-1,4-Dimercapto-2,3-butandiol -Lösung, DTT
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About This Item
Empfohlene Produkte
Qualität
for molecular biology
Qualitätsniveau
Produktlinie
BioUltra
Assay
900-1100 mmol/L (Titration molarity)
Form
liquid
Eignung der Reaktion
reagent type: reductant
Konzentration
~1 M in H2O
Verunreinigungen
DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected
pH-Wert
2.7-5.0
Dichte
1.04 g/mL at 20 °C
Absorption
cut-off at 330 nm
Eignung
in accordance for filter test
suitable for molecular biology
Lagertemp.
2-8°C
SMILES String
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChIKey
VHJLVAABSRFDPM-QWWZWVQMSA-N
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Allgemeine Beschreibung
Anwendung
- bei der Behandlung von Darmexplantaten[3]
- bei der Homogenataufbereitung von Kiemenzellen für enzymatische Assays[4]
- bei der Proteinelektrophorese für Western Blotting[5]
- bei der Transglutaminaseaktivität[6]
- im Probenpuffer für SDS-PAGE[7]
- in Western Blots als Bestandteil des Lysepuffers für die Einzelkern-RNA-Sequenzierung[8]
- als Bestandteil von Proteinextraktionspuffer für die Zelllyse[8]
Biochem./physiol. Wirkung
Leistungsmerkmale und Vorteile
- DTT-Lösung 1 mol/l in BioUltra-Qualität für den Einsatz in der Molekularbiologie
- frei von DNasen, RNasen, Phosphatasen und Proteasen
Sonstige Hinweise
Vergleichbares Produkt
Ähnliches Produkt
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Dam. 1 - Skin Irrit. 2
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How stable is this DTT solution ? it is known that DTT is unstable in solution and should be kept under inert atmosphere. How long can I keep it once open ?
1 answer-
This product is a prepared solution of 1M DTT in water. In the original sealed container it is quite stable, with an assigned retest schedule of every 2 years. Assuming that the product is handled properly, the material is expected to be stable until at least the retest date reported on the lot specific Certificate of Analysis.
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