Atto 550 hat eine molekulare Absorption von 120.000 und eine Quantenausbeute von 80% in Wasser, so dass er starke Fluoreszenz zeigt. Die Abklingzeit der Fluoreszenz beträgt 3.2 ns.
Anwendung
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3™, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies.
Rechtliche Hinweise
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
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Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
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