33247

Harnstoff

ACS reagent grade, ≥99%, solid, puriss. p.a., reag. Ph. Eur.

• Synonym(e): Carbamid, Carbonyldiamid
• Lineare Formel: NH₂CONH₂
• CAS-Nummer: 57-13-6
• Molekulargewicht: 60.06
• NACRES: NA.21
• PubChem Substance ID: 57648752
• UNSPSC Code: 12352100
• EC Number: 200-315-5
• MDL number: MFCD00008022
• Beilstein/REAXYS Number: 635724
• Assay: ≥99%, 99-101% (calc. on dry substance)
• Form: solid

Eigenschaften

• Produktname: Harnstoff, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%
• Quality Segment: 200
• agency: reag. Ph. Eur.
• grade: ACS reagent, puriss. p.a.
• assay: ≥99%, 99-101% (calc. on dry substance)
• form: solid
• impurities: alkalic reac. substances, complies, ≤0.0005% heavy metals (as Pb), ≤0.05% ammonium (NH₄), ≤0.1% biuret
• ign. residue: ≤0.01% (as SO₄)
• loss: ≤1.0% loss on drying, 105°C, 1 h
• mp: 132-135 °C
• solubility: H₂O: soluble 480 g/L at 20 °C
• density: 1.335 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤5 mg/kg, sulfate (SO₄^2-): ≤10 mg/kg
• cation traces: Fe: ≤2 mg/kg
• suitability: in accordance for appearance of solution, in accordance for identity
• functional group: amine
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• Gene Information: human ... CA1(759), CA2(760) ; rat ... Ppm1a(24666)

Beschreibung

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.

Urea is the most commonly used slow release nitrogen fertilizer.

Application

Urea has been used in the following studies:

• Synthesis of SnO₂ nanoparticles.[1]
• Synthesis of urethanes, ureides and semicarbazides.
• Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain.[2]
• Protein electrophoretic studies.[3]

Wird für die Denaturierung von Proteinen und als mildes Mittel zur Solubilisierung unlöslicher oder denaturierter Proteine?? verwendet. Nützlich für die Renaturierung von Proteinen aus Proben, die bereits mit 6 M Guanidinchlorid vergällt sind, wie z.B. Einschlüssen. Kann mit Guanidin-hydrochlorid und Dithiothreitrol (DTT) für die Rückfaltung von denaturierten Proteinen in ihre native oder aktive Form eingesetzt werden.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)