11209264001

DNA Molecular Weight Marker VII

pkg of 50 μg (in 200 μl), solution

• Synonym(e): DNA marker
• UNSPSC-Code: 41105335

Eigenschaften

• Form: solution
• Qualitätsniveau: 100
• Verpackung: pkg of 50 μg (in 200 μl)
• Hersteller/Markenname: Roche
• Konzentration: 250 μg/mL
• Farbe: colorless
• Löslichkeit: water: miscible
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Contents
Ready-to-use solution, 250 μg/ml, in TE buffer (Tris-HCl, 1 mM EDTA, pH 8.0).

Fragment mixture prepared by cleavage of SPP1-DNA with restriction endonuclease EcoR I.
The product is also available as DNA Molecular Weight Marker VII, Digoxigenin-labeled.
Size Range: 0.081 to 8.57 kbp

Anwendung

DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

• Restriction digests
• PCR
• RT-PCR

Sequenz

The mixture contains seventeen fragments with the following base pair lengths (1 base pair = 660 daltons): 81, 359, 492, 710, 718*, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899, 6106, 7427, and 8576 as determined by computer analysis of the SSP1 sequence.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
*Alonso, J.C. and Trautner, T.A., personal communication of unpublished results, the 718-bp fragment is present in non-stoichometric amounts.

Einheitendefinition

50 μg = 1A₂₆₀ unit

Angaben zur Herstellung

Storage conditions (working solution): 2 to 8 °C
Once thawed store at 2 to 8 °C.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash