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Merck

PHCC360KIT

Scepter 3.0 Automatischer Zellzähler im Pipettenformat

Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter 3.0 Cell Counter Sensors

Scepter 3.0 Handheld Automated Cell Counter

Synonym(e):

Cell counting instrument


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€ 4.360,00

€ 4.360,00


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NACRES:
NC.56
UNSPSC Code:
41131501

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manufacturer/tradename

Scepter

technique(s)

cell counting: suitable

particle size

6-36 μm

compatibility

for use with cells sized 4.0–25.0 μm

shipped in

ambient

storage temp.

no temp limit

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Dieser Artikel
PHCC340KITPHCC360500PHCC340050
compatibility

for use with cells sized 4.0–25.0 μm

compatibility

for use with cells sized 4.0–25.0 μm

compatibility

Scepter for use with cells sized 8.0–25.0 μm

Not compatible with Scepter 2.0 Instruments

compatibility

Scepter for use with cells sized 5.0–15.0 μm

Not compatible with Scepter 2.0 Instruments

technique(s)

cell counting: suitable

technique(s)

cell counting: suitable

technique(s)

cell counting: suitable

technique(s)

cell counting: suitable

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

manufacturer/tradename

Scepter

manufacturer/tradename

Scepter

manufacturer/tradename

Scepter

manufacturer/tradename

Scepter

particle size

6-36 μm

particle size

4-18 μm

particle size

6-36 μm

particle size

4-18 μm

General description

Der automatische Zellzähler Scepter 3.0 bietet eine schnelle Methode für die Zellzählung in einem gebrauchsfreundlichen Pipettenformat. Der Scepter 3.0 ist nur für Forschungszwecke vorgesehen und wurde mit Zelltypen geprüft, die den heutzutage genutzten entsprechen. Nach Herstellung einer Verdünnung der betreffenden Zellkultur wird der Anwender mithilfe von Anweisungen auf dem Display durch die Probennahme geführt. Weniger als 30 Sekunden, nachdem der Scepter Zellzähler die Probe in den Scepter Zellzähler-Sensor aufgesaugt hat, werden auf dem Scepter Zellzähler-Display folgende Werte angezeigt:

  • Zellkonzentration
  • Durchschnittliche Zellgröße
  • Durchschnittliches Zellvolumen
  • Histogramm der Größen- oder Volumenverteilung
Das Gerät speichert bis zu 999 Zählungen, die auf den Computer des Anwenders heruntergeladen und für weitere Datenbearbeitung hochgeladen werden können.

Der Scepter 3.0 Zellzähler ist sowohl mit dem 40-µM- als auch mit dem 60-µM-Zellzähler-Sensor des Scepter 3.0 kompatibel. In diesem Kit (PHCC360KIT) ist eine Gratispackung mit 60-μm-Sensoren enthalten (50 Stück).
Upgrading to Wi-Fi connectivity and Millicell® Cloud Access:

Scepter 3.0 instruments purchased before January 1st, 2026, do not have Wi-Fi connectivity. Customers can add Wi-Fi connectivity and get cloud connectivity using the following steps:

1. Download the free software at Millicell® Cloud. Select Scepter 3.0 and download the software onto a USB drive. Load this Software onto the Scepter 3.0 device.

2. Place an order for free trial subscription of Millicell® Cloud using the Catalog number MILLICL-TRIAL. Contact our customer service to help you with this.

3. Purchase Wi-Fi dongle: MERS03USBD0N. Insert the USB dongle into the USB port and follow the instructions on the screen. Now your device is ready to access Millicell® Cloud.

Other Notes

  • 1 Scepter 3.0 automatischer Zellzähler im Pipettenformat
  • 1 Test-Bead-Fläschchen
  • 1 Ladestation
  • 50 Sensoren (60 μm)
Der Scepter 3.0 Zellzähler ist mit den Sensoren der vorherigen Generation nicht kompatibel; er ist nur mit den unter „Verwandte Produkte“ aufgeführten Scepter 3.0 Zellzähler-Sensoren kompatibel.

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklasse

10-13 - German Storage Class 10 to 13

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Protokolle

Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.

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Questions

1–4 of 4 Questions  
  1. Can I confirm if the sensors are single-use only please?

    1 answer
    1. The Scepter 3.0 sensors are single use only.

      Helpful?

  2. What is the maximum size of cell clumps that can pass through the Scepter 3.0? Also, what size screen is used to break up the cell clump aggregates in both Scepter 2.0 and 3.0?

    1 answer
    1. The maximum size of cell clumps that can pass through the Scepter 3.0 is determined by the aperture limits, which are 40um and 60um for sensors 40 and 60, respectively. Unlike the Scepter 2.0, the Scepter 3.0 does not use a screen to break up cell clump aggregates; instead, it employs a set of pillars to achieve this function. In contrast, the Scepter 2.0 utilizes a 35um Nylon filter to break up cell clump aggregates.

      Helpful?

  3. Is this appropriate for size 15-23 μm cell? Does the Kit include also the charger? How many 60/40 μm sensors are included Which sensors can be used with this instrument? Which sensors are appropriate for cells size 15-23 μm and what is their price?

    1 answer
    1. Please see the answers to the following questions below:

      1. Is this appropriate for size 15-23 μm cells?
      Yes, the Scepter 3.0 is suitable. As listed on the product detail page in the DESCRIPTION section for use with cells sized 4.0–25.0 μm.

      2. Does the Kit include the charger?
      This product does include 60μM sensors and a charging station.

      3. How many 60/40 μm sensors are included
      As mentioned on the product detail page in the DESCRIPTION section under 'Components', it includes 50 Sensors.

      4. Which sensors can be used with this instrument?
      This product is only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products on this page. Please see the link below to review additional information regarding the Scepter 3.0:
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/scepter-cell-counter

      5. Which sensors are appropriate for cells size 15-23 μm and what is their price?
      See question 1, this product is suitable for the cell size of 15-23 μm.

      Regarding pricing, Please navigate to the link https://www.sigmaaldrich.com/support/customer-support, and click on "Products Pricing & Availability" under the Products Section with all the required information so that a member of the Customer Service team can reach out to assist further. Thank you.

      Helpful?

  4. Does it require Trypan blue or another dye? Does it distinguish between live and dead cells?

    1 answer
    1. The Scepter is a particle counter, but it will not differentiate live from dead cells. The device can be used to gauge cell health on the basis of cell size. For example, in cell types where apoptotic cells are smaller in size compared to non-apoptotic cells. This shift in size can be quantified using the device option "Edit Histogram" to either gate around this population or exclude the population from the count.

      Please see the following manual below for more information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/799/484/00110230w-rev1020-ms.pdf

      Helpful?

Reviews

Active Filters

  1. R. Moldova
    • Review 1
    • Votes 4
    3 out of 5 stars.

    Suggestion

    I could have been more careful when ordering the Scepter 3.0 components (Handheld Automated Cell Counter and Cell sensors), because without experience I ordered sensors not suitable for this type of reader. That is, instead of PHCC360500
    Cell Counter Sensors I ordered PHCC60500.
    No one raised any questions. Regrettable. I will have to incur additional expenses.
    I really hope to fix this mistake to be able to apply this device in practice.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for reaching out and sharing your review with us. We strive to provide the information customers need to make a confident purchase. Your feedback is extremely valuable in helping us to update the product information for this product to clarify which sensors are compatible with the Scepter 3.0.

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