The minimum purity of this product is 95% as determined by SDS-PAGE. The purity is confirmed in the Certificate of Analysis. Please see the link below to review a lot-specific Certificate:
https://www.sigmaaldrich.com/product/mm/324681#product-documentationZ#product-documentation
324681
Elastase, menschliches Neutrophil
Elastase, Human Neutrophil, CAS 9004-06-2, is a serine protease that degrades elastin, collagen, and proteoglycans
Synonym(e):
Elastase, menschliches Neutrophil
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 50 μg | Warenkorb auf Verfügbarkeit prüfen | € 418,00 | |
| 100 μg | Warenkorb auf Verfügbarkeit prüfen | € 645,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.77
MDL number:
Specific activity:
≥20 units/mg protein
Technischer Dienst
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Unterstützung erhaltenQuality Segment
description
Merck USA index - 14, 3535
form
lyophilized solid
specific activity
≥20 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
ambient
storage temp.
−20°C
General description
Elastase, menschliches Neutrophil, CAS-Nr. 9004-06-2, ist eine Serinprotease, die Elastin, Collagen und Proteoglykane abbaut.
Native Elastase von menschlichem Neutrophil. Baut Collagen, Elastin und Proteoglykane ab. Elastase wurde mit der Entstehung von Lungenemphysem und rheumatoider Arthritis in Verbindung gebracht.
Native Elastase von menschlichem Neutrophil. Eine Serinprotease, die Elastin, Collagen und Proteoglykane abbaut. Deren Überexpression wurde mit der Entstehung von Lungenemphysem, rheumatoider Arthritis und zystischer Fibrose in Verbindung gebracht.
Physical form
Salzfrei.
Preparation Note
Aus Plasma hergestellt, das in zertifizierten Tests für HBsAg und Antikörper gegen HIV und HCV als negativ befunden wurde.
In 50 mM Natriumacetat, pH 5,5, mit 200 mM NaCl oder in anderen wässrigen Puffern rekonstituieren. Das Material ist bei fast neutralem pH-Wert am aktivsten, doch für die Erhaltung der Stabilität nach der Rekonstitution und Vermeidung einer Autolyse sollte ein leicht saurer Puffer (pH 5,5) verwendet werden.
Nach der Rekonstitution in Aliquote aufteilen und für die langfristige Lagerung einfrieren (-20 °C) oder für die kurzfristige Lagerung im Kühlschrank (4 °C) aufbewahren. Saure Stammlösungen sind bei einer Temperatur von 4 °C bis zu 1 Monat und bei einer Temperatur von -20 °C bis zu 1 Jahr haltbar.
Analysis Note
Hauptbande mittels SDS-PAGE.
Other Notes
Eine Einheit ist definiert als die Menge an Enzym, die 1,0 µmol MeO-Suc-Ala-Ala-Pro-Val-pNA (Kat. Nr. 454454) pro Minute bei 25 °C und pH 8,0 hydrolisiert.
Siedle, B., et al. 2002. Biorg. Med. Chem.10, 2855.
Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
Selak, M.A. 1992. Thromb. Haemost.68, 570.
Baugh, R.J., and Travis, J. 1976. Biochemistry15, 836.
Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
Selak, M.A. 1992. Thromb. Haemost.68, 570.
Baugh, R.J., and Travis, J. 1976. Biochemistry15, 836.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxizität: Standard-Handhabung (A)
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥20 units/mg protein | specific activity ≥50 units/mg protein (Bradford) | specific activity ≥25 units/mg protein | specific activity ≥2 units/mg protein |
| form lyophilized solid | form lyophilized powder | form lyophilized | form lyophilized solid (Salt-free) |
| storage condition OK to freeze | storage condition - | storage condition OK to freeze, desiccated | storage condition OK to freeze |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in ambient | shipped in dry ice | shipped in ambient | shipped in ambient |
| manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Lagerklasse
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Verwandter Inhalt
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 324681-100UG | 07790788059583 |
| 324681-50UG | 04055977196825 |
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Could you provide the purity data of item Elastase, Human Neutrophil, cat# 324681? Thank you.
1 answer-
Helpful?
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How do I calculate how much enzyme powder I need to dissolve to hydrolyze the 290 mg of protein contained in the solution?
1 answer-
I am not sure which substrate you are using. The unit definition for this product is One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-pNA (Cat. No. 454454) per min at 25°C, pH 8.0.
This product has unit activity specification as ≥20 units/mg protein. If for example the results are
20 units/mg, this means to use 1 unit you will need to use 50 ug of the elastase.
You might wish to refer to the Elastase activity assay at:Helpful?
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Hi Is this form of neutrophil elastase active and suitable be used directly in a cell assay? And is it sterile ? Mvh Magdalena
1 answer-
This form of neutrophil elastase is a salt-free preparation and is supplied in an active form with a specific activity of ≥20 units/mg protein. It is not sterile and has not been tested for cell culture applications. To use it in a cell culture assay, sterile filtration is necessary, which may result in some product retention on the filter.
Helpful?
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Has this product been tested for the presence of endotoxin?
1 answer-
Unfortunately, this product has not been tested for the presence for endotoxins
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what is the molecular wight of the enzyme ?
1 answer-
Unfortunately, the molecular weight has not been determined for this product. A number of estimates have been reported in the literature. Please see the link below to a publication that may be helpful:
https://www.sciencedirect.com/science/article/pii/S1016847823175091?via%3DihubHelpful?
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Is this the active form of the enzyme? or does it require activation by cathepsin?
1 answer-
Here are the recommended reconstitution and reaction conditions:
Solubility information:
Reconstitute in 50 mM sodium acetate, pH 5.5, with 200 mM NaCl or in other aqueous buffers. Material is most active near a neutral pH, but to maintain stability upon reconstitution, use in a slightly acidic buffer (pH 5.5) to avoid autolysis.
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.Human Neutrophil Elastase Protocol
1. Dissolve enzyme in reaction buffer: 100 mM Tris-HCl, pH 7.5, 500 mM NaCl.
2. Prepare the substrate stock solution by dissolving Elastase Substrate I (MeOSuc-Ala-Ala-Pro-Val-pNA, Cat. No. 324696) at 50 mM in DMSO.
3. Just prior to assay, dilute the substrate stock solution with an equal volume of 200 mM Tris-HCl, pH 8.0. Mix well.
4. Prepare the assay mixture by combining 960 µl of reaction buffer with 40 µl of diluted substrate stock solution.
5. Add enzyme to assay mixture, mix, and incubate at 25°C.
6. Monitor the change in absorbance at 410 nm. Use an ε of 8800 at 410 nm to calculate the hydrolysis of nitroanilide.Helpful?
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