We have not tested pH on this and do not have a working temperature range on this product. However, we do mention: One unit will liberate 1.0 micromole of glucose from cellulose per hour at pH 5.0 at 37 deg C (2 hour assay).
C0615
Cellulase from Trichoderma sp.
powder, ≥5,000 units/g solid
Synonym(s):
Onozuka RS
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 g | Please contact Customer Service for Availability | $343.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-734-4
MDL number:
eCl@ss:
32160410
Specific activity:
≥5,000 units/g solid
form
powder
Quality Level
specific activity
≥5,000 units/g solid
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
solubility
deionized water: soluble 3.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB))
greener alternative category
storage temp.
2-8°C
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This Item | |||
|---|---|---|---|
| specific activity ≥5,000 units/g solid | specific activity ≥1 unit/mg solid | specific activity ≥700 units/g | specific activity ≥1000 units/g |
| form powder | form lyophilized powder | form aqueous solution | form solid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility deionized water: soluble 3.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB)) | solubility deionized water: soluble 5.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB).) | solubility - | solubility - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
General description
Manufactured by Yakult
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research
Application
Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries.[1] Cellulase is added to detergents to improve cleansing properties.[1] Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose while investigating the biodegradability of bioabsorbable bacterial cellulose (BBC).[2]
Biochem/physiol Actions
Preparation Note
Dissolves in sterile deionized (DI) water in the presence of 0.15 polyhexamethylene biguanide (PHMB) at 3 mg/mL concentration.
Other Notes
One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Youwen Pan et al.
Applied and environmental microbiology, 76(5), 1433-1441 (2010-01-06)
Biofilm formation by Listeria monocytogenes is generally associated with its persistence in the food-processing environment. Serotype 1/2a strains make up more than 50% of the total isolates recovered from food and the environment, while serotype 4b strains are most often
Gaku Tokuda et al.
Biology letters, 3(3), 336-339 (2007-03-22)
The intestinal flagellates of termites produce cellulases that contribute to cellulose digestion of their host termites. However, 75% of all termite species do not harbour the cellulolytic flagellates; the endogenous cellulase secreted from the midgut tissue has been considered a
A Brant et al.
Occupational and environmental medicine, 61(9), 793-795 (2004-08-20)
Three employees from two different detergent companies were investigated for occupational asthma, using skin prick tests, serum specific IgE, and specific bronchial challenge. Two were challenged with lipase and one with cellulase. All three cases had immunological evidence of sensitisation
Rey Allen et al.
Applied and environmental microbiology, 85(7) (2019-02-03)
Phototrophic biofilms are key to nutrient cycling in natural environments and bioremediation technologies, but few studies describe biofilm formation by pure (axenic) cultures of a phototrophic microbe. The cyanobacterium Synechocystis sp. strain PCC 6803 (here Synechocystis) is a model microorganism
Yang Hu et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 97(1), 114-123 (2011-02-04)
Cellulose biodegradation resulting from enzymolysis generally occurs in nature rather than in the human body because of the absence of cellulose degrading enzymes. In order to achieve in-vivo degradation in human body for in-vivo tissue regeneration applications, we developed a
Protocols
To standardize an enzymatic assay procedure of cellulase.
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C0615-1G | 04061835555765 |
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What is the working pH and temperature, and the optimal pH and temperature, please?
1 answer-
Helpful?
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¿What cellulases does the lyophilized powder C0615 contain? and ¿what is the ratio?
1 answer-
This product is a high purity, high activity cellulase derived from Trichoderma viride. An isoenzyme profile has not been performed. The link below may be useful:
https://febs.onlinelibrary.wiley.com/doi/full/10.1111/j.1432-1033.1985.tb08653.xHelpful?
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Does product C0615, cellulase from Trichoderma viride, contain any sugar as a stabilizer?
1 answer-
No. Product C0615, cellulase from Trichoderma viride, does not contain any sugar stabilizer.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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