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重要文件

TOX6

Sigma-Aldrich

体外毒理学检测试剂盒,基于磺酰罗丹明B

同義詞:

biomass viability assay, sulforhodamine B, total biomass assay

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.84
暫時無法取得訂價和供貨情況

形狀

liquid

用途

1 kit sufficient for 1,000 tests

包裝

pkg of 1 kit

儲存條件

dry at room temperature

λmax

565 nm

應用

cell analysis
detection

檢測方法

colorimetric

儲存溫度

room temp

一般說明

磺酰罗丹明B方法简便、准确,结果一致。该分析系统是一种测量总生物量的方法,通过染色细胞蛋白与硫血红蛋白B。

應用

基于磺酰罗丹明B的体外毒性检测试剂盒已被用于通过总蛋白进行生物质(活细胞和死细胞)的分光光度测定。[1][2]它用于研究17-烯丙基氨基-17-去甲氧格尔德霉素 (17-AAG) 对五种葡萄膜黑色素瘤细胞系增生的作用。[3]在565nm波长处测量染料的吸光度。

生化/生理作用

染料与细胞蛋白结合并随后溶解在碱中。

訊號詞

Danger

危險聲明

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

儲存類別代碼

3 - Flammable liquids


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分析證明 (COA)

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P Skehan et al.
Journal of the National Cancer Institute, 82(13), 1107-1112 (1990-07-04)
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
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Santos F, eta l.
Endocrine-related cancer, 21(1), 1-40 (2014)
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PloS one, 9(9), e107109-e107109 (2014-09-23)
Epithelial ovarian carcinoma (EOC), the major cause of gynaecological cancer death, is a heterogeneous disease classified into five subtypes. Each subtype has distinct clinical characteristics and is associated with different genetic risk factors and molecular events, but all are treated
Wei Gao et al.
American journal of cancer research, 10(6), 1710-1727 (2020-07-10)
Oral tongue squamous cell carcinoma (OTSCC) has a distinctive cell sub-population known as tumor-initiating cells (TICs). CD271 is a functional TIC receptor in head and neck cancers. The molecular mechanisms governing CD271 up-regulation remains unclear. Oxidative stress is a contributing
Yue-mei Ma et al.
Journal of experimental & clinical cancer research : CR, 28, 23-23 (2009-02-21)
Recent data have redefined the concept of inflammation as a critical component of tumor progression. However, there has been little development on cases where inflammation on or near a wound and a tumor exist simultaneously. Therefore, this pilot study aims

文章

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

以細胞為基礎的細胞增殖 (BrdU、MTT、WST1)、細胞活力和細胞毒性實驗,應用於癌症、神經科學和幹細胞研究。

Questions

1–3 of 3 Questions  
  1. Can you use Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based with suspension cells?

    1 answer
    1. Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B can be used with both adherent and suspension culture.The advantage of layering the fixative directly onto the media is that this allows the assay to work for cells in suspension.  Otherwise, you need to centrifuge, remove the media and then fix.  Also, it is possible to loose cells when you remove the media. It was found that adding the fixative works for the assay and the protocol was changed in 2003.

      Helpful?

  2. When using Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based, what happens if I add kit component Product T5413, 10 mM Tris Base Solution, prior to fixing the cells?

    1 answer
    1. If the 10 mM Tris Base Solution, Product T5413 is added to the cells before the TCA (fixative), the cells will be lysed.  This reagent is not buffered and is not isotonic. The assay cannot be rescued if this occurs.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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