Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B can be used with both adherent and suspension culture.The advantage of layering the fixative directly onto the media is that this allows the assay to work for cells in suspension. Otherwise, you need to centrifuge, remove the media and then fix. Also, it is possible to loose cells when you remove the media. It was found that adding the fixative works for the assay and the protocol was changed in 2003.
推薦產品
形狀
liquid
用途
1 kit sufficient for 1,000 tests
包裝
pkg of 1 kit
儲存條件
dry at room temperature
λmax
565 nm
應用
cell analysis
detection
檢測方法
colorimetric
儲存溫度
room temp
訊號詞
Danger
危險聲明
危險分類
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A
儲存類別代碼
3 - Flammable liquids
從最近期的版本中選擇一個:
分析證明 (COA)
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Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
以細胞為基礎的細胞增殖 (BrdU、MTT、WST1)、細胞活力和細胞毒性實驗,應用於癌症、神經科學和幹細胞研究。
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Can you use Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based with suspension cells?
1 answer-
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When using Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based, what happens if I add kit component Product T5413, 10 mM Tris Base Solution, prior to fixing the cells?
1 answer-
If the 10 mM Tris Base Solution, Product T5413 is added to the cells before the TCA (fixative), the cells will be lysed. This reagent is not buffered and is not isotonic. The assay cannot be rescued if this occurs.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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